A modified Neuhoff's colloidal Coomassie Blue G‐250 stain is reported, dubbed “blue silver”
on account of its considerably higher sensitivity, approaching the one of conventional silver …

Glomerular targets of autoimmunity in human membranous nephropathy are poorly understood.
Here, we used a combined proteomic approach to identify specific antibodies against …

Even if nephrotic syndrome is characterized by massive urinary loss of major plasma proteins,
a clear structural characterization based on proteomics has never been reported. Urine …

Objective. Neutrophil extracellular traps (NET) expose modified antigens for autoantibodies
in vasculitis. Little is known about levels and removal pathways of NET in systemic lupus …

The release of soluble ligands of activating Natural Killer (NK) cell receptors may represent
a regulatory mechanism of NK cell function both in physiologic and in pathologic conditions. …

NETs constitute a network of DNA and proteins released by neutrophils in response to
infectious and immunologic triggers.NET proteins are recognized as autoantigens in ANCA …

Exosomes are secreted nanovesicles that are able to transfer RNA and proteins to target
cells. The emerging role of mesenchymal stem cell (MSC) exosomes as promoters of aerobic …

Annexin A1 is a protein with multifunctional roles in innate and adaptive immunity mainly
devoted to the regulation of inflammatory cells and the resolution of inflammation. Most of the …

Ultracentrifugationon sucrose density gradientappears to be the best purification protocol
for extracellular vesicle (EVs) purification. After this step, to reduce disulfide bridges linking …

Results A total of 2950 proteins were isolated from microvesicles and exosomes, including
1579 (54%) identified in all samples but only 178 (6%) and 88 (3%) specific for medullary …