Possible involvement of oxidative stress in salivary gland of patients with Sjogren's syndrome

Pathobiology. 2006;73(5):252-60. doi: 10.1159/000098211.

Abstract

Objective: To determine the involvement of oxidative stress in the salivary gland of patients with Sjogren's syndrome (SS).

Methods: Oxidative damage to the gland was measured by 8-hydroxy-2'-deoxyguanosine (8-OHdG) and hexanoyl-lysine (HEL) using the SS saliva. In addition, lactate dehydrogenase (LDH) and mitochondrial glutamic-oxaloacetic transaminase (m-GOT), both general markers for cell damage, were also analyzed.

Results: Increased levels of 8-OHdG and HEL were found in the saliva of SS patients, but not in that of patients with other salivary gland dysfunction or of healthy individuals. Levels of LDH and m-GOT were significantly correlated with 8-OHdG and HEL levels, respectively. Furthermore, the increased levels of 8-OHdG and HEL were also correlated in the SS saliva.

Conclusion: These findings suggested the involvement of oxidative stress in glandular tissue destruction in SS. It was indicated that the detection of 8-OHdG and HEL in the saliva may become a useful tool for the diagnosis of SS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Adult
  • Aged
  • Aged, 80 and over
  • Aspartate Aminotransferases / analysis
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / analysis
  • Female
  • Humans
  • L-Lactate Dehydrogenase / analysis
  • Middle Aged
  • Mitochondria / metabolism
  • Oxidative Stress / physiology*
  • Saliva / chemistry
  • Salivary Glands / metabolism*
  • Sjogren's Syndrome / diagnosis*
  • Sjogren's Syndrome / metabolism*

Substances

  • 8-Hydroxy-2'-Deoxyguanosine
  • L-Lactate Dehydrogenase
  • Aspartate Aminotransferases
  • Deoxyguanosine