Abstract
A physical map of the CXC chemokine locus on chromosome 4 has been constructed by PCR analysis and PFGE mapping of YAC clones. The genes for IL8, GRO1, PPBP, PF4, SCYB5 (ENA-78) and SCYB6 (GCP-2) have been co-localized on a 335-kb genomic fragment. The GRO2 and GRO3 genes did not map within this region and based on analysis of a YAC contig overlapping IL8 we speculate that GRO2 and GRO3 map downstream of this region. We have also assigned the novel CXC chemokine gene, SCYB9B (alias H174/betaR1) to chromosome 4q21, upstream and within 12 kb of INP10. Like INP10 and MIG, INP10 and SCYB9B are arranged in a head to tail manner. The chromosomal arrangement of these genes appears to reflect the evolution of this multigene family and supports the theory that it arose by gene duplication.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biological Evolution
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Chemokine CXCL1
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Chemokine CXCL5
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Chemokine CXCL6
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Chemokines, CXC / genetics*
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Chemotactic Factors / genetics
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Chromosomes, Artificial, Yeast / genetics
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Chromosomes, Human, Pair 4 / genetics*
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Electrophoresis, Gel, Pulsed-Field
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Gene Duplication
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Growth Substances / genetics
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Humans
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Intercellular Signaling Peptides and Proteins*
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Interleukin-8 / analogs & derivatives
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Interleukin-8 / genetics
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Multigene Family
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Phylogeny
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Physical Chromosome Mapping
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Platelet Factor 4 / genetics
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Polymerase Chain Reaction
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Restriction Mapping
Substances
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CXCL1 protein, human
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CXCL3 protein, human
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CXCL5 protein, human
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CXCL6 protein, human
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Chemokine CXCL1
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Chemokine CXCL5
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Chemokine CXCL6
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Chemokines, CXC
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Chemotactic Factors
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Growth Substances
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Intercellular Signaling Peptides and Proteins
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Interleukin-8
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Platelet Factor 4