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A new class of membrane-bound chemokine with a CX3C motif

Abstract

Chemokines direct the trafficking of white blood cells in immune surveillance, playing a key role in inflammatory and infectious diseases such as AIDS1–5. All chemokines studied so far are secreted proteins of relative molecular mass 7K–15K and fall into three families that are defined by a cysteine signature motif: CXC, CC and C (refs 3, 6, 7), where C is a cysteine and X any amino-acid residue. We report here the identification and characterization of a fourth human chemokine type, derived from non-haemopoietic cells and bearing a new CX3C fingerprint. Unlike other chemokine types, the polypeptide chain of the human CX3C chemokine is predicted to be part of a 373-aminoacid protein that carries the chemokine domain on top of an extended mucin-like stalk. This molecule can exist in two forms: either membrane-anchored or as a shed 95K glycoprotein. The soluble CX3C chemokine has potent chemoattractant activity for T cells and monocytes, and the cell-surface-bound protein, which is induced on activated primary endothelial cells, promotes strong adhesion of those leukocytes. The structure, biochemical features, tissue distribution and chromosomal localization of CX3C chemokine all indicate that it represents a unique class of chemokine that may constitute part of the molecular control of leukocyte traffic at the endothelium.

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References

  1. Strieter, R. M. et al. J. Immunol. 156, 3583–3586 (1996).

    CAS  PubMed  Google Scholar 

  2. Baggiolini, M. & Dahinden, C. A. Immunol. Today 104, 27–29 (1994).

    Google Scholar 

  3. Schall, T. J. & Bacon, K. B. Curr. Opin. Immunol. 6, 865–873 (1994).

    Article  CAS  Google Scholar 

  4. Weiss, R. A. & Clapham, P. R. Nature 381, 647–648 (1996).

    Article  ADS  CAS  Google Scholar 

  5. Pemack, B. P. & Schall, T. J. Nature Med. 2, 1174–1178 (1996).

    Article  Google Scholar 

  6. Clore, G. M. & Gronenborn, A. M. FASEB J. 9, 57–62 (1995).

    Article  CAS  Google Scholar 

  7. Wells, T. N. et al. J. Leuk. Biol. 59, 53–60 (1996).

    Article  CAS  Google Scholar 

  8. Altschul, S. F., Boguski, M. S., Gish, W. & Wootton, J. C. Nature Genet. 6, 119–129 (1994).

    Article  CAS  Google Scholar 

  9. Kelner, G. S. et al. Science 266, 1395–1399 (1994).

    Article  ADS  CAS  Google Scholar 

  10. Lennon, G., Auffray, C., Polymeropoulos, M. & Soares, M. B. Genomics 33, 151–152 (1996).

    Article  CAS  Google Scholar 

  11. Gendler, S. J. & Spicer, A. P. Annu. Rev. Physiol. 57, 607–634 (1995).

    Article  CAS  Google Scholar 

  12. Hansen, J. E. et al. Biochem. J. 308, 601–813 (1995).

    Article  Google Scholar 

  13. Shimizu, Y. & Shaw, S. Nature 366, 630–631 (1993).

    Article  ADS  CAS  Google Scholar 

  14. Bernfield, M. et al. Annu. Rev. Cell. Biol. 8, 365–393 (1992).

    Article  CAS  Google Scholar 

  15. Vyas, P., Vickers, M. A., Picketts, D. J. & Higgs, D. R. Genomics 29, 679–689 (1995).

    Article  CAS  Google Scholar 

  16. Springer, T. A. Cell 76, 301–314 (1994).

    Article  CAS  Google Scholar 

  17. Lasky, L. A. Science 258, 964–969 (1992).

    Article  ADS  CAS  Google Scholar 

  18. Butcher, E. C. & Picker, L. J. Science 272, 60–66 (1996).

    Article  ADS  CAS  Google Scholar 

  19. Webb, L. M. C., Ehrengruber, M. U., Clark-Lewis, I., Baggiolini, M. & Rot, A. Proc. Natl Acad. Sci. USA 90, 7158–7162 (1993).

    Article  ADS  CAS  Google Scholar 

  20. Tanaka, Y., Adams, D. H. & Shaw, S. Immunol. Today 361, 79–82 (1993).

    CAS  Google Scholar 

  21. Witt, D. P. & Lander, A. D. Curr. Biol. 4, 394–400 (1994).

    Article  CAS  Google Scholar 

  22. Schall, T. J., Bacon, K., Toy, K. J. & Goeddel, D. V. Nature 347, 669–671 (1990).

    Article  ADS  CAS  Google Scholar 

  23. Dawson, P. E., Muir, T. W., Clark-Lewis, I. & Kent, S. B. Science 266, 776–779 (1994).

    Article  ADS  CAS  Google Scholar 

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Bazan, J., Bacon, K., Hardiman, G. et al. A new class of membrane-bound chemokine with a CX3C motif. Nature 385, 640–644 (1997). https://doi.org/10.1038/385640a0

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