Expression of interleukin-17B in mouse embryonic limb buds and regulation by BMP-7 and bFGF

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Abstract

Interleukin-17B (IL-17B) is a member of interleukin-17 family that displays a variety of proinflammatory and immune modulatory activities. In this study, we found that IL-17B mRNA was maximally expressed in the limb buds of 14.5 days post coitus (dpc) mouse embryo and declined to low level at 19.5 dpc. By immunohistochemical staining, the strongest IL-17B signals were observed in the cells of the bone collar in the primary ossification center. The chondrocytes in the resting and proliferative zones were stained moderately, while little staining was seen in the hypertrophic zone. Furthermore, in both C3H10T1/2 and MC3T3-E1 cells, the IL-17B mRNA was up-regulated by recombinant human bone morphogenetic protein-7, but down-regulated by basic fibroblast growth factor via the extracellular signal-regulated kinase pathway. This study provides the first evidence that IL-17B is expressed in the mouse embryonic limb buds and may play a role in chondrogenesis and osteogenesis.

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Materials and methods

Mouse embryos and neonatal mice. This study was approved by the Institutional Animal Care and Use Committee of the University of California, Davis, according to National Institutes of Health guidelines. Timed pregnant CD-1 mice were obtained from the Charles River Laboratories (Wilmington, MA) and kept at the animal facilities of University of California, Davis. The pregnant mice from 9.5 to 17.5 dpc and neonatal mice born at 19.5 dpc were euthanized by CO2 asphyxiation. Embryos and neonatal mice

IL-17B mRNA was expressed in mouse embryonic limb buds

IL-17 cytokines form a unique novel family, of which the prototype member IL-17A has been studied extensively [1], [2], [3], [4], [5], [6]. However, reports on IL-17B were very limited [10], [11], [12], [21]. Our finding that IL-17B was expressed in calf articular cartilage but not in adult cow cartilage led us to hypothesize that IL-17B is perhaps predominantly expressed in early stages of bone development. To test our hypothesis, we isolated total RNA from mouse embryonic limb buds and limbs

Acknowledgments

We thank Dr. T.K. Sampath, Creative BioMolecules, Hopkinton, MA, for his generous gift of BMP-7. This study was supported by a grant from the National Institutes of Health (5R01AR047345-02).

References (32)

  • E.E. Moore et al.

    Expression of IL-17B in neurons and evaluation of its possible role in the chromosome 5q-linked form of Charcot-Marie-Tooth disease

    Neuromuscul. Disord.

    (2002)
  • Z. You et al.

    c-Myc sensitizes cells to tumor necrosis factor-mediated apoptosis by inhibiting nuclear factor kappa B transactivation

    J. Biol. Chem.

    (2002)
  • J. Laurikkala et al.

    Identification of a secreted BMP antagonist, ectodin, integrating BMP, FGF, and SHH signals from the tooth enamel knot

    Dev. Biol.

    (2003)
  • P.J. Marie

    Fibroblast growth factor signaling controlling osteoblast differentiation

    Gene

    (2003)
  • J. Witowski et al.

    Interleukin-17: a mediator of inflammatory responses

    Cell. Mol. Life Sci.

    (2004)
  • L.K. Stamp et al.

    Interleukin-17: the missing link between T-cell accumulation and effector cell actions in rheumatoid arthritis?

    Immunol. Cell Biol.

    (2004)
  • Cited by (0)

    Abbreviations: IL-17, interleukin-17; BMP-7, bone morphogenetic protein-7; bFGF, basic fibroblast growth factor; ERK, extracellular signal-regulated kinase; Sef, similar expression to fgf genes; PTHrP, parathyroid hormone-related protein.

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