Original investigationAssessment of Unspecific Near-Infrared Dyes in Laser-Induced Fluorescence Imaging of Experimental Arthritis
Section snippets
NIR Dyes
Two NIR dyes were investigated: an aqueous solution of ICG (Pulsion GmbH, Munich, Germany) and a solution of the hydrophilic carbocyanine derivative 1,1′-bis-(4-sulfobutyl) indotricarbocyanine-5,5′-dicarboxylic acid diglucamide monosodium salt (SIDAG; Schering AG, Berlin, Germany). ICG is a hydrophilic anionic dye of which up to 98% is bound to plasma proteins as early as 2 seconds after IV injection. Its median lethal dose in mice ranges from 60 to 80 μmol/kg (17). The dye is excreted solely
Kinetics of NIR Fluorescence Dyes
After injection of ICG, healthy animals showed a rapid increase in NFI of the ankle joints for both dosages, with AUCs of (15 ± 3) count * s (A1) and (19 ± 8) count * s (A2). The subsequent slow decrease had a slope of –0.0032 ± 0.0001 FI units (FIU)/s (A1) and –0.0029 ± 0.0001 FIU/s (A2; Figure 2).
After injection of SIDAG, there was a slow increase in NFI throughout the interval of 150 seconds investigated, with an AUC of (220 ± 60) count * s (B1) and (290 ± 90) count * s (B2).
Animals with
Discussion
The study aims at whether normal and inflammatory joints can be differentiated by means of the pharmacokinetic behavior of nonspecific dyes, determined by means of fluorescence imaging. More specifically, the experimental study was conducted to investigate two nonspecific NIR dyes in terms of their pharmacokinetic behavior in normal and inflammatory ankle joints of mice.
The animal model of Lyme arthritis used in this study is based on the assumption that systemic inflammation is induced by
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2016, Best Practice and Research: Clinical RheumatologyCitation Excerpt :The different inflammatory patterns and various shapes of ICG fluorescence enhancement may offer opportunities to distinguish RA from osteoarthritis. Dynamic ICG imaging provides opportunities for quantitative therapy monitoring and potential distinction between responders and nonresponders to therapy [158]. In preclinical settings, non-FDA-approved dyes have also been investigated for the detection of arthritis.
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2015, Computerized Medical Imaging and GraphicsCitation Excerpt :For instance, ICG has the ability to bind both high-density and low-density lipoproteins, relevant to the characteristics of its lipophilic [18]. In fact, ICG could enable in vivo detection of inflamed atheroma, lipid-rich, human macrophages and human atherosclerosis [19–21]. In the last few years, several IVPA imaging systems have become available for imaging atherosclerosis [2–5].
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