Prostaglandin D2 metabolite stimulates collagen synthesis by human osteoblasts during calcification

https://doi.org/10.1016/0090-6980(91)90001-VGet rights and content

Abstract

We investigate the effect of the prostaglandin D2 metabolite Δ12−PGD2 (9−Deoxy−Δ9, Δ12−13,14-dihydroprostaglandin D2) on collagen synthesis in human osteoblast. Δ12-PGJ2 at 10−5M enhanced collagen synthesis in the presence of 2 mM α-glycerophosphate-2Na. The stimulative effect appeared as early as 3 days after addition and continued until 22 days. The enhancement of type I collagen synthesis was confirmed by polyacrylamide gel electrophoresis. The potency was the same as 101t-8M 1 α, 25 dihydroxy vitamine D3 (1,25(OH)2D3). Northern blot analysis showed that 10−5M Δ 12-PGD2 and 10−8M 1,25(OH)2D3 enhanced the transcribtion of type 1 procollagen (α1) mRNA levels in osteoblasts.

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