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Galactosylation of IgG from rheumatoid arthritis (RA) patients – changes during therapy

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Abstract

It is well documented that serum IgG from rheumatoid arthritis (RA) patients exhibits decreased galactosylation of its conservative N-glycans (Asn-297) in CH2 domains of the heavy chains; it has been shown that this agalactosylation is proportional to disease severity. In the present investigation we analyzed galactosylation of IgG derived from the patients using a modified ELISA-plate test, biosensor BIAcore and total sugar analysis (GC-MS). For ELISA and BIAcore the binding of IgG preparations, purified from the patients’ sera, to two lectins: Ricinus communis (RCA-I) and Griffonia simplicifolia (GSL-II) was applied. Based on ELISA-plate test an agalactosylation factor (AF, a relative ratio of GSL-II/RCA-I binding) was calculated, which was proportional to actual disease severity. Repeated testing of several patients before and after treatment with methotrexate (MTX) alone or in combination with Remicade (a chimeric antibody anti-TNF-α) supplied results indicating an increase of IgG galactosylation during the treatment. This introductory observation suggests that IgG galactosylation may be an additional indicator of the RA patients’ improvement.

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Abbreviations

IgG:

immunoglobulin G

RA:

rheumatoid arthritis

RCA:

lectin from Ricinus communis

PVL:

lectin from Psathyrella velutina

GSL:

lectin from Griffonia simplicifolia

ESR:

erythrocyte sedimentation rate

CRP:

C-reactive protein

GC-MS:

gas chromatography-mass spectrometry

MTX:

methotrexate

NC:

nitrocellulose

DTT:

dithiotreitol

AF:

agalactosylation factor

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Authors and Affiliations

  1. Ludwik Hirszfeld Institute of Immunology & Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, 53-114, Wroclaw, Poland

    Marta Pasek, Maria Duk, Maria Podbielska, Elwira Lisowska & Hubert Krotkiewski

  2. Department of Rheumatology, Medical University, Wisniowa 36, 53-137, Wroclaw, Poland

    Renata Sokolik & Jacek Szechiński

Authors
  1. Marta Pasek
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  2. Maria Duk
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  3. Maria Podbielska
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  4. Renata Sokolik
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  5. Jacek Szechiński
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  6. Elwira Lisowska
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  7. Hubert Krotkiewski
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Correspondence to Hubert Krotkiewski.

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Pasek, M., Duk, M., Podbielska, M. et al. Galactosylation of IgG from rheumatoid arthritis (RA) patients – changes during therapy. Glycoconj J 23, 463–471 (2006). https://doi.org/10.1007/s10719-006-5409-0

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  • DOI: https://doi.org/10.1007/s10719-006-5409-0

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