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Analysis of Carbonylated Proteins in Bronchoalveolar Lavage of Patients with Diffuse Lung Diseases

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Abstract

Diffuse lung diseases (DLD) are a heterogeneous group of diseases with different etiopathogenesis, clinical course, and prognosis. It has been demonstrated that oxidative stress can contribute to the pathogenesis of these diseases. In the present study we measured carbonylated protein concentrations in the BAL of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis, idiopathic pulmonary fibrosis, and for the first time in patients with chronic eosinophilic pneumonia and extrinsic allergic alveolitis. Our aim was to further investigate oxidation products in diffuse lung diseases. Oxidatively modified protein concentrations were increased in the BAL of patients than in that of controls (0.22 nmol/mg protein vs 0.05 nmol/mg protein; p < 0.001) and in each group of disease versus controls, suggesting that proteins that have become dysfunctional by oxidation could play a role in the pathogenesis of diffuse lung diseases. Further studies in a greater number of patients are needed to understand the contribution of oxidatively modified proteins to the pathogenesis of DLD and, in particular, to the development of extrinsic allergic alveolitis where the highest levels of carbonylated proteins were found.

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Abbreviations

BAL:

bronchoalveolar lavage

DLD:

diffuse lung diseases

S:

sarcoidosis

SSc:

pulmonary fibrosis associated with systemic sclerosis

IPF:

idiopathic pulmonary fibrosis

EAA:

extrinsic allergic alveolitis

CEP:

chronic eosinophilic pneumonia

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Acknowledgments

This study was supported by Ministry of University and Research (MIUR), Italy. The authors thank Dr. Collodoro for performing the bronchoscopies with bronchoalveolar lavages and Dr. Refini for lung function tests.

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Authors and Affiliations

  1. Respiratory Diseases Section, Department of Clinical Medicine and Immunological Sciences, Siena University, 53100, Siena, Italy

    Elena Bargagli, Francesco Penza, Cecilia Vagaggini, Maria Grazia Perari & Paola Rottoli

  2. Molecular Biology, Department Molecular Biology, Siena University, Siena, Italy

    Barbara Magi

Authors
  1. Elena Bargagli
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  2. Francesco Penza
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  3. Cecilia Vagaggini
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  4. Barbara Magi
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  5. Maria Grazia Perari
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  6. Paola Rottoli
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Correspondence to Elena Bargagli.

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Bargagli, E., Penza, F., Vagaggini, C. et al. Analysis of Carbonylated Proteins in Bronchoalveolar Lavage of Patients with Diffuse Lung Diseases. Lung 185, 139–144 (2007). https://doi.org/10.1007/s00408-007-9001-6

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  • DOI: https://doi.org/10.1007/s00408-007-9001-6

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