Abstract
The human monoclonal autoantibody 16/6 is a common anti-DNA idiotype found to have clinical relevance in patients with systemic lupus erythematosus (SLE). Therefore the ability of peripheral blood T cells of SLE patients and healthy controls to proliferate and to produce helper T-cell factors following stimulation with this idiotype was tested. It was found that T cells of 75% of healthy donors proliferated to the 16/6 idiotype, whereas only 22% of SLE patients responded to this idiotype by proliferation. On the other hand, the capability to produce T-cell helper factors specific to the 16/6 idiotype was found in a higher percentage of SLE patients (48%) as compared to healthy controls (31%). The low frequency of proliferative responses in SLE patients might be due either to the chronic exposure to the 16/6 idiotype or to the production of antiidiotype antibodies against the 16/6 idiotype, which interfere with the response to the latter stimulator.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bresnihan B, Jasin HE: Suppressor function of peripheral blood mononuclear cells in normal individuals and in patients with SLE. J Clin Invest 59:106–116, 1977
Krakauer RS, Globugh JP, Frank S, Sundeen JT: Suppressor cell function defect in idiopathic systemic lupus erythematosus. Clin Immunol Immunopathol 14:327–333, 1979
Ginsburg WW, Finkelman FD, Lipsky PE: Circulating and pokeweed mitogen-induced immunoglobulin secreting cells in systemic lupus erythematosus. Clin Exp Immunol 35:76–88, 1979
Gottlieb AB, Lahita RG, Chiorazzi N, Kunkel HG: Immune function in systemic lupus erythematosus: impairment of in vitro T cell proliferation and in vivo antibody response to exogenous antigen. J Clin Invest 63:885–892, 1979
Williams GW, Steinberg AD, Reinertsen JL, Klassen LW, Decker JL, Dollin R: Influenza immunization in systemic lupus erythematosus. A double blind trial. Ann Intern Med 88:729–734, 1978
Katz D, Bentwich Z, Eshhar N, Löwy I, Mozes E: Immune response potential to poly(Tyr,Glu)-poly(DLAla)-poly(Lys) of human T cells of different donors. Proc Natl Acad Sci USA 78:4505–4509, 1981
Suez D, Katz D, Brautbar C, Cohen T, Weisman Z, Bentwich Z, Mozes E: HLA-linked immune responsiveness to (T,G)-A-L: A family study. Hum Immunol 13:219–234, 1985
Shalev Y, Bentwich Z, Katz D, Brautbar C, Mozes E: (T,G)-A-L specific immune response potential and HLA-typing of Israel patients with systemic lupus erythematosus. Clin Exp Immunol 60:355–362, 1985
Schwartz RS, Stollar BD: Origins of anti-DNA autoantibodies. J Clin Invest 75:321–327, 1985
Shoenfeld Y, Hsu Lin SC, Gabriels JE, Silberstein BC, Furie BC, Furie B, Stollar BD, Schwartz RS: Production of autoantibodies by human-human hybridomas. J Clin Invest 70:205–208, 1982
Shoenfeld Y, Rauch J, Massicotte H, Datta SK, Andre-Schwartz J, Stollar BD, Schwartz RS: Polyspecificity of monoclonal lupus autoantibodies by human hybridoma. N Engl J Med 308:414–420, 1983
Shoenfeld Y, Isenberg DA, Rauch J, Madaio MP, Stollar BD, Schwartz RS: Idiotypic cross-reactions of monoclonal human lupus autoantibodies. J Exp Med 158:718–730, 1983
Isenberg DA, Shoenfeld Y, Madaio MP, Rauch J, Reichlin M, Stollar BD, Schwartz RS: Anti-DNA antibody idiotypes in SLE. Lancet 2:417–422, 1984
Isenberg DA, Collins C: Detection of cross-reactive anti-DNA antibody idiotypes on renal tissue-bound immunoglobulins from lupus patients. J Clin Invest 76:287–294, 1985
Isenberg D, Dudeney D, Wojnaruska F, Bhogal BS, Rauch J, Schattner A, Naparstek Y, Duggan D: Detection of cross-reactive anti-DNA antibody idiotypes on tissue-bound immunoglobulins from skin biopsies of lupus patients. J Immunol 135:261–264, 1985
Tan EM, Cohen AS, Fries JF, Masi AT, McShane DJ, Rothfield NF, Green Schaller J, Talal N, Winchester RJ: The 1982 revised criteria for the classification of systemic lupus erythematosus. Arth Rheum 25:1271–1277, 1982
Morrow WJW, Isenberg DA, Todd-Pokpropek A, Parry HF, Snaith ML: Useful laboratory measurements in the management of systemic lupus erythematosus. Q J Med 202:125–138, 1982
Isenberg DA, Shoenfeld Y, Schwartz RS: Multiple serologic reactions and their relationship to clinical activity in SLE. Arth Rheum 27:132–138, 1984
Adar R, Papa MZ, Halpern Z, Mozes M, Shoshan S, Sofer B, Zinger H, Dayan M, Mozes E: Cellular sensitivity to collagen in thromboangitis obliterans. N Engl J Med 308:1113–1116, 1983
Mage MG, McHugh LL, Rothstein TL: Mouse lymphocyte with and without surface immunoglobulin preparation scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin. J Immunol Methods 15:47–56, 1977
Crains E, St. Germain J, Bell DA: The in vitro production of anti-DNA antibody by cultured peripheral blood or tonsillar lymphoid cells from normal donors and SLE patients. J Immunol 135:3839–3844, 1985
Datta SK, Naparstek Y, Schwartz RS: In vitro production of an anti-DNA idiotype by lymphocytes of normal subjects and patients with SLE. Clin Immunol Immunopathol 38:302–318, 1981
Abdou N: The idiotype anti-idiotype network in human autoimmunity. J Clin Immunol 5:365–369, 1985
Takeuchi T, Hosomo O, Koido J, Homma M, Abe T: Suppression of rheumatoid factor synthesis by anti-idiotypic antibody in rheumatoid arthritis patients with cross-reactive idiotypes. Arth Rheum 28:873–881, 1985
Dang H, Fischbach M, Talal N: Anti-idiotypic antiserum to monoclonal anti-Sm inhibits the autoantigen-induced proliferative response. J Immunol 134:3825–3830, 1985
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mendlovic, S., Shoenfeld, Y., Bakimer, R. et al. In vitro T-cell functions specific to an anti-DNA idiotype and serological markers in patients with systemic lupus erythematosus (SLE). J Clin Immunol 8, 178–187 (1988). https://doi.org/10.1007/BF00917564
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00917564