Summary
Circulating immune complexes (CIC) were isolated from sera of 35 patients with rheumatoid arthritis (RA) by a two-step method using 2% polyethylene glycol precipitation and anti-C1q affinity chromatography. By this method CIC were exclusive to 19 patients with vasculitis, nodules, or Sjögren's syndrome. Levels of CIC did not correlate with the severity of synovitis but reflected the extent of extra-articular disease. Furthermore, in four patients with persistent severe synovitis observed over a period of 4 to 16 months, the levels of CIC paralleled changes in extra-articular disease. Despite such additional evidence, whether the relationship between CIC and tissue injury is causative or consequential remains unresolved.
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Lambert PH, Dixon FJ, Zubler RH, Agnello V, Cambiaso C, Casali P, Clarke J, Cowdery JS, McDuffie FC, Hay FC, MacLennan ICM, Masson P, Muller-Eberhard HJ, Pentinnen K, Smith M, Tappeiner G, Theofilopoulos AN, Verroust P (1978) A WHO collaborative study for the evaluation of eighteen methods for detecting immune complexes in serum. J Clin Lab Immunol 1:1–15
Conn DL, McDuffie FC, Dyck PJ (1972) Immunopathologic study of sural nerves in rheumatoid arthritis. Arthritis Rheum 15:135–145
Nowoslawski A (1971) Immunopathological features of rheumatoid arthritis. In: Muller W, Harwerth HG, Fehr K (eds) Rheumatoid arthritis. Academic Press, London New York, pp 325–334
Bourke BE, Moss IK, Mumford PA, Horsfall AC, Maini RN (1982) The complement fixing ability of putative circulating immune complexes in rheumatoid arthritis and its relationship to extra-articular disease. Clin Exp Immunol 48:726–732
Erhardt CC, Mumford P, Maini RN (1979) The association of cryoglobulinaemia with nodules, vasculitis and fibrosing alveolitis in rheumatoid arthritis and their relationship to serum C1q binding activity and rheumatoid factor. Clin Exp Immunol 38:405–413
Mumford PA, Horsfall AC, Maini RN (1982) The frequency of circulating immune complexes in rheumatoid arthritis and systemic lupus erythematosus. Rheumatol Int 1:181–186
Nydegger, UE, Zubler RH, Gabay R, Joliat G, Karagevrekis CH, Lambert PH, Miescher PA (1977) Circulating complement breakdown products in patients with rheumatoid arthritis. J Clin Invest 59:862–868
Zubler RH, Nydegger U, Perrin LH, Fehr K, McCormick J, Lambert PH, Miescher PA (1976) Circulating and intra-articular immune complexes in patients with rheumatoid arthritis. J Clin Invest 57:1308–1319
Sokoloff L, McCluskey RT, Bunim JJ (1953) Vascularity of the early subcutaneous nodule of rheumatoid arthritis. AMA Arch Pathol 55:475–495
Mongan ES, Cass RM, Jacox RF, Vaughan JH (1969) A study of the relation of seronegative and seropositive rheumatoid arthritis to each other and to necrotising vasculitis. Am J Med 47:23–35
Pallis CA, Scott JT (1965) Peripheral neuropathy in rheumatoid arthritis. Br Med J 1:1141–1147
Lawley TJ, Moutsopoulos HM, Katz SI, Theofilopoulos AN, Chused TM, Frank MM (1979) Demonstration of circulating immune complexes in Sjögren's syndrome. J Immunol 123:1382–1387
Yoshinoya S, McDuffy S, Alarcon-Segovia D, Pope RM (1982) Detection and partial characterisation of immune complexes in patients with rheumatoid arthritis plus Sjögren's syndrome and with Sjögren's syndrome alone. Clin Exp Immunol 48:339–347
A Committee of the American Rheumatism Association (1959) Diagnostic criteria for rheumatoid arthritis 1958 revision. Ann Rheum Dis 18:49–53
Ritchie DM, Boyle JA, McInnes JM, Jasani MK, Dalakos TG, Crieveson P, Buchannan WW (1968) Clinical studies with an articular index for the assessment of joint tenderness in patients with rheumatoid arthritis. Q J Med 37:393–406
Yonemasu K, Stroud RM (1971) C1q: rapid purification method for preparation of monospecific antisera and for biochemical studies. J Immunol 106:304–313
Chapman JR, Charles PJ, Venables PJW, Thompson PJ, Haslam PL, Maini RN, Turner-Warwick MEH (1984) Definition and clinical relevance of antibodies to nuclear ribonucleoprotein and other nuclear antigens in patients with cryptogenic fibrosing alveolitis. Am Rev Respir Dis 130:439–443
Melsom RD (1984) Clinical correlation and immunochemical analysis of immune complexes in rheumatoid arthritis. MD thesis, University of Cambridge
Soltis RD, Hasz DE (1983) The effect of serum immunoglobulin concentration on immune complex detection by polyethylene glycol. J Immunol Methods 57:275–282
Hack CE, Eerenberg-Belmer AJ, Hannema AJ, Out TA, Aalberse RC (1981) Polyethylene glycol enhances the binding of C1q to circulating immune complexes. J Immunol Methods 44:211–221
Melsom RD, Smith PR, Maini RN (1986) Demonstration of an unidentified 48 kD polypeptide in circulating immune complexes in rheumatoid arthritis. Ann Rheum Dis (in press)
Theofilopoulos AN, Dixon FJ (1979) The biology and detection of immune complexes. Adv Immunol 28:89–220
Venables PJW, Erhardt CC, Maini RN (1980) Antibodies to extractable nuclear antigens and rheumatoid arthritis: relationship to vasculitis and circulating immune complexes. Clin Exp Immunol 39:146–153
Hannestad K, Johanessen A (1976) Polyclonal antibodies to IgG (rheumatoid factors) which cross-react with cell nuclei. Scand J Immunol 5:541–547
Male DK, Roitt IM (1981) Molecular analysis of complement fixing rheumatoid synovial fluid immune complexes. Clin Exp Immunol 46:521–529
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Melsom, R.D., Horsfall, A.C., Schrieber, L. et al. Anti-Clq affinity isolated circulating immune complexes correlate with extra-articular rheumatoid disease. Rheumatol Int 6, 227–231 (1986). https://doi.org/10.1007/BF00541372
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DOI: https://doi.org/10.1007/BF00541372