Summary
The effects of soluble monosodium urate (350, 50, and 5 μmol/l) on the proliferation and proteoglycan synthesis of lapine articular chondrocytes were studied in a monolayer culture system. Cellular proliferation in vitro was unaffected by urate treatment. Chondrocytes treated with 50 μmol/l sodium urate showed a 26% reduction in binding of 35SO4 to matrix macromolecules. However, this reduction did not achieve statistical significance. The 350 and 5 μmol/l concentrations did not alter 35SO4 binding. It is proposed that monosodium urate has no marked direct effect on chondrocyte viability, proliferation and proteoglycan synthesis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Schumacher HR, Phelps P (1971) Sequential changes in human polymorphonuclear leukocytes after urate crystal phagocytosis: An electron microscopic study. Arthritis Rheum 14:513–526
Spilberg I (1975) Current concepts of the mechanism of acute inflammation in gouty arthritis. Arthritis Rheum 18:129–134
Hoffstein S, Weissmann G (1975) Mechanism of lysosomal enzyme release from leukocytes. IV. Interaction of monosodium urate crystals with dogfish and human leukocytes. Arthritis Rheum 18:153–165
Boss GR, Seegmiller JE (1979) Hyperuricemia and gout. Classification, complications and management. N Engl J Med 300:1459–1468
Hasselbacher P (1979) C 3 activation by monosodium urate monohydrate and other crystalline material. Arthritis Rheum 22:571–578
Hasselbacher P, McMillan RM, Vater CA, Hahn J, Harris ED Jr (1981) Monosodium urate monohydrate (MSUM) crystals stimulate secretion of collagenase and PGE2 by synovial fibroblasts: a model of joint destruction in chronic gouty arthritis (Abstr). Arthritis Rheum 24:S 77
Ali SY, Evans L (1973) Enzymic degradation of cartilage in osteoarthritis. Fed Proc 32:1494–1498
Sapolsky AI, Keiser H, Howell DS, Woessner JF (1976) Metalloproteases of human articular cartilage that digest cartilage proteoglycan at neutral and acid pH. J Clin Invest 58:1030–1041
Dingle JT (1979) Recent studies on the control of joint damage: the contribution of the Strangeways Research Laboratory. Ann Rheum Dis 38:201–214
Green WT Jr (1971) Behavior of articular chondrocytes in cell culture. Clin Orthop 75:248–260
Sokoloff L, Malemud CJ, Green WT Jr (1970) Sulfate incorporation by articular chondrocytes in monolayer culture. Arthritis Rheum 13:118–124
Fratantoni JC, Hall CW, Neufeld EF (1968) The defect in Hurler's and Hunter's syndromes: faulty degradation of mucopolysaccharide. Proc Natl Acad Sci USA 60:699–706
Corvol M-T, Malemud CJ, Sokoloff L (1972) A pituitary growth-promoting factor for articular chondrocytes in monolayer culture. Endocrinology 90:262–271
Burton K (1956) A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. J Biochem 62:315–323
Kruskal WH (1952) A non-parametric test for the several sample problem. Ann Math Stat 23:525–540
Nemenyi P (1963) Distribution-free multiple comparisons. State University of New York, Downstate Medical Center, New York
Sokoloff L (1957) The pathology of gout. Metabolism 6: 230–243
Uehlinger E (1976) Pathologische Anatomie der Gicht. Verh Dtsch Ges Rheumatol 4:95–101
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kirkpatrick, C.J., Mohr, W. & Haferkamp, O. The effect of soluble sodium urate on the proliferation and proteoglycan synthesis of lapine articular chondrocytes in monolayer culture. Rheumatol Int 1, 131–133 (1981). https://doi.org/10.1007/BF00541257
Issue Date:
DOI: https://doi.org/10.1007/BF00541257