Abstract
The objective of this study was to measure soluble intercellular adhesion molecule-1 (sICAM-1) in patients with Behçet's disease (BD) and to analyse the relationship of sICAM-1 levels with clinical and some laboratory measures of disease activity. Forty patients with BD fulfilling the International Study Group Criteria for the diagnosis of BD and 20 healthy controls were studied. Twenty patients had active, and 20 patients had inactive disease. Serum sICAM-1 was determined by a sandwich ELISA. The mean (±SD) sICAM-1 level was significantly higher in the whole BD group (297.3±86.6 ng/ml) than in the healthy controls (213±83.5 ng/ml; P< 0.05). The mean sICAM-1 levels in active and inactive BD patients were 315.7±76.3 ng/ml and 258.3±73.3 ng/ml, respectively. The mean sICAM-1 level in active patients was significantly higher than in inactive patients and healthy controls (P< 0.02 and P< 0.001, respectively). No statistically significant difference in mean sICAM-1 levels was found between inactive BD patients and healthy controls (P>0.05). There was no statistically significant difference between the mean sICAM-1 levels of active patients with (351.3±77.2 ng/ml) or without vascular lesions (292±68.8; P>0.05). In spite of a positive correlation between disease activity and both erythrocyte sedimentation rate and C-reactive protein (CRP; P< 0.01), we found no correlation between sICAM-1 and either of them (P>0.05). The elevated levels of sICAM-1 may be due to endothelial cell activation and/or damage or may be the result of inflammation. In either case it did not seem to be superior to more conventional measures of disease activity.
Similar content being viewed by others
References
Rothlein R, Dustin ML, Marlin SD, Springer TA (1986) A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. J Immunol 137:1270–1274
Marlin SD, Springer SA (1987) Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1). Cell 51:813–819
Staunton DE, Marlin SD, Stratowa C, Dustin ML, Springer TA (1988) Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families. Cell 52:925–933
Springer T (1900) Adhesion receptors of the immune system. Nature 346:425–434
Smith ME, Thomas JA (1990) Cellular expression of lymphocyte function associated antigens and the intercellular adhesion molecule-1 in normal tissue. J Clin Pathol 43:893–900
Rothlein R, Czajkowski M, O'Neill MM, Marlin SD, Mainolfi E, Merluzzi VJ (1988) Induction of intercellular adhesion molecule 1 on primary and continuous cell lines by proinflammatory cytokines: regulation by pharmacologic agents and neutralizing antibodies. J Immunol 141:1665–1669
Dustin ML, Rothlein R, Bhan AK, Dinarello CA, Springer TA (1986) Induction by IL-1 and interferon gamma: tissue distribution, biochemistry and function of a natural adherence molecule (ICAM-1). J Immunol 137:245–254
Pober JS, Lapierre LA, Stolpen AH, Brock TA, Springer TA, Fiers W, Bevilacqua MP, Mendrick DL, Gimbrone MA (1987) Activation of cultured human endothelial cells by recombinant lymphotoxin: comparison with tumor necrosis factor and interleukin 1 species. J Immunol 138:3319–3324
Smith CW, Marlin SD, Rothlein R, Toman C, Anderson DC (1989) Cooperative interactions of LFA-1 and Mac-1 with intercellular adhesion molecule-1 in facilitating adherence and transendothelial migration of human neutrophils in vitro. J Clin Invest 83:2008–2017
Dougherty GJ, Murdoch S, Hogg N (1988) The function of human intercellular adhesion molecule-1 (ICAM-1) in the generation of an immune response. Eur J Immunol 18:35–39
Seth R, Raymond FD, Makgoba MW (1991) Circulating ICAM-1 isoforms: diagnostic prospects for inflammatory and immune disorders. Lancet 338:83–84
Ballantyne C, Mainolfi EA, Young JB, Windsor NT, Cocanougher B, Lawrence EC, Anderson DC, Rothlein R (1991) Prognostic value of increased levels of circulating intercellular adhesion molecule-1 after heart transplant. Clin Res 39:285 a
Rothlein R, Mainolfi EA, Czajkowski M, Marlin SD (1991) A form of circulating ICAM-1 in human serum. J Immunol 147:3788–3793
Adams DH, Mainolfi E, Burra P, Neuberger JM, Ayres R, Elias E, Rothlein R (1992) Detection of circulating intercellular adhesion molecule-1 in chronic liver diseases. Hepatology 16:810–814
Furukawa S, Imai K, Matsubara T, Yone K, Yachi A, Okumura K, Yabuta K (1992) Increased levels of circulating intercellular adhesion molecule-1 in Kawasaki disease. Arthritis Rheum 35:672–677
Shijubo N, Imai K, Aoki S, Hirasawa M, Sugawara H, Koba H, Tsujisaki M, Sugiyama T, Hinoda Y, Yachi A, Asakawa M, Suzuki A (1992) Circulating intercellular adhesion molecule-1 antigen in sera of patients with idiopathic pulmonary fibrosis. Clin Exp Immunol 89:58–62
Cush JJ, Rothlein R, Lindsley HB, Mainolfi EA, Lipsky PE (1993) Increased levels of circulating intercellular adhesion molecule 1 in the sera of patients with rheumatoid arthritis. Arthritis Rheum 36:1098–1102
Mason JC, Kapahi P, Haskard DO (1993) Detection of increased levels of circulating intercellular adhesion molecule in some patients with rheumatoid arthritis but not in patients with systemic lupus erythematosus. Arthritis Rheum 4:519–527
Sfikakis PP, Tesar J, Baraf H, Lipnick R, Klipple G, Tsokos GC (1993) Circulating intercellular adhesion molecule-1 in patients with systemic sclerosis. Clin Immunol Immunopathol 68:88–92
Hauschild S, Schmitt WH, Kekow J, Szymkowiak C, Gross WL (1992) Elevated serum levels of ICAM-1 in Wegener's granulomatosis. Immun Infect 20:84–85
Machold KP, Kiener HP, Graninger W, Graninger WB (1993) Soluble intercellular adhesion molecule-1 in patients with rheumatoid arthritis and systemic lupus erythematosus. Clin Immunol Immunopathol 68:74–78
Mizushima Y (1986) Chemotaxis and phagocytosis of leucocytes in Behçet's disease: an overview. In: Lehner T, Barnes CG (eds) Recent advances in Behçet's disease. Royal Society of Medicine Services, London, pp 85–86
Jorizzo JL, Solomon AR, Cavalho T (1985) Behçet's syndrome, immunopathologic and histologic assessment of pathergy lesions is useful in diagnosis and follow-up. Arch Pathol Lab Med 109:745–751
Chun S, Su DWP, Lee S (1990) Histopathologic study of cutaneous lesions in Behçet's syndrome. J Dermatol 17:333–341
International Study Group for Behçet's Disease (1990) Criteria for the diagnosis of Behçet's disease. Lancet 335:1078–1080
Aydintuĝ AO, Tokgöz G, Jedryka-Goral A, D'Cruz D, Gürler A, Khamashta MA, Hughes GRV (1993) Von Willebrand factor antigen in patients with Behçet's disease. In: Wechsler B, Godeau P (eds) Behçet's disease (International congress series). Elsevier, Amsterdam, pp 131–133
Aydintuĝ AO, Tokgöz G, D'Cruz DP, Gürler A, Cervera R, Düzgün N, Atmaca LS, Khamashta MA, Hughes GRV (1993) Antibodies to endothelial cells in patients with Behçet's disease. Clin Immunol Immunopathol 67:157–162
Hamzaoui K, Ayed K, Slim A, Hamza M, Touraine J (1990) Natural killer cell activity, interferon gamma and antibodies to herpes viruses in patients with Behçet's disease. Clin Exp Immunol 79:28–34
Hamzaoui K, Hamza M, Ayed K (1990) Production of TNF-alpha and IL-1 in active Behçet's disease. J Rheumatol 17:1428–1429
Mege J, Dilşen N, Sanguedolce V, Gül A, Bongrand P, Roux H, Öcal L, Inanç M, Capo C (1993) Overproduction of monocyte derived tumor necrosis factor alpha, interleukin (IL) 6, IL-8 and increased neutrophil superoxide generation in Behçet's disease. A comparative study with familial mediterranean fever and healthy subjects. J Rheumatol 20:1544–1549
Gül A, Esin S, Dilşen N, Wigzell H, Biberfeld P (1993) Immunohistology of skin pathergy reaction in Behçet's disease. In: Wechsler B, Godeau P (eds) Behçet's disease. (International congress series). Elsevier, Amsterdam, pp 343–347
Aoki S, Imai K, Yachi A (1993) Soluble intercellular adhesion molecule-1 (ICAM-1) antigen in patients with rheumatoid arthritis. Scand J Immunol 38:485–490
Cronstein BN, Weissmann G (1993) The adhesion molecules of inflammation. Arthritis Rheum 36:147–157
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Aydintuĝ, A.O., Tokgöz, G., Özoran, K. et al. Elevated levels of soluble intercellular adhesion molecule-1 correlate with disease activity in Behçet's disease. Rheumatol Int 15, 75–78 (1995). https://doi.org/10.1007/BF00262712
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00262712