Selective Deimination of Vimentin in Calcium Ionophore-Induced Apoptosis of Mouse Peritoneal Macrophages

doi:10.1006/bbrc.1998.8148

Biochemical and Biophysical Research Communications

Volume 243, Issue 3, 24 February 1998, Pages 641-646

https://doi.org/10.1006/bbrc.1998.8148 Get rights and content

Abstract

We found citrulline-containing proteins in mouse peritoneal macrophages undergoing calcium ionophore-induced apoptosis. Such proteins were products of deimination of arginine residues catalyzed by endogenous peptidylarginine deiminase (EC 3.5.3.15) activated by calcium influx. Western blotting analyses of the extract from macrophages incubated with 1 μM ionomycin showed selective deimination of vimentin without detectable degradation. Double immunofluorescence staining of deiminated proteins and vimentin suggested localization of deiminated vimentin around the periphery of round-shaped nucleus, which was thought to be an early morphological sign of apoptosis. The biological implication of vimentin deimination in macrophage apoptosis is discussed.

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Regular ArticleSelective Deimination of Vimentin in Calcium Ionophore-Induced Apoptosis of Mouse Peritoneal Macrophages

Abstract

Biochim. Biophys. Acta

Anal. Biochem.

Cell Biol. Int.

J. Biol. Chem.

J. Biol. Chem.

J. Invest. Dermatol.

Biochem. Biophys. Res. Commun.

J. Biol. Chem.

J. Biol. Chem.

J. Immunol.

Regular Article
Selective Deimination of Vimentin in Calcium Ionophore-Induced Apoptosis of Mouse Peritoneal Macrophages