Table 3.

Studied cell subsets analyzed by flow cytometry.

Cell Subsets	SLE			HC
Cell Subsets	N	Median	1st–3rd Quartile	N	Median	1st–3rd Quartile
Cells/µl
Leukocytes	17	5900	4800–8800	20	6400	5575–7450
Lymphocytes	15	762.2	542.2–870.7	20	1443.3^****	1190.6–1948.5
T lymphocytes	15	599.9	393.4–723.3	20	1001^****	830.2–1252.4
Th lymphocytes	15	376.4	150.3–408.5	20	618.1^****	492.1–687.3
Tc lymphocytes	16	235.3	162.7–325.7	20	299.2^*	269.4–409.5
CD4−CD8− T lymphocytes	14	22.9	14.4–33.0	20	91.9^****	57.7–104.4
HLA-DR+ T lymphocytes	16	80.4	57.6–128.6	20	135.7	70.1–252.2
Th1-like lymphocytes	7	49.35	24.6–173.40	19	80.19 ^*	65.92–102.10
Th2-like lymphocytes	7	16.75	9.26–28.90	20	18.19	14.49–26.07
Th1Th17-like lymphocytes	7	17.23	12.00–30.90	20	55.53^**	39.42–69.02
Th17-like lymphocytes	7	15.59	11.23–18.08	20	21.99	13.46–32.99
aTreg lymphocytes	11	4.64	3.37–6.87	20	10.44^***	8.61–16.93
rTreg lymphocytes	11	6.51	2.03–10.48	20	8.49^*	6.21–11.36
Treg lymphocytes	11	11.13	6.69–17.01	20	23.35^**	16.20–25.93
non-Treg lymphocytes	11	15.91	8.11–40.20	20	15.69	12.30–22.87
FOXP3+ Th lymphocytes	11	26.46	16.98–52.95	20	30.57	26.25–42.03
Tcon lymphocytes	11	349.90	137.9–383.8	20	583.2^***	465.3–650.1
Percentages
Leukocytes
Lymphocytes	17	15.700	8.000–18.800	20	22.597^**	17.735–28.008
Lymphocytes
T lymphocytes	17	76.700	69.400–81.300	20	67.100^**	63.975–74.300
T lymphocytes
Th lymphocytes	17	54.000	45.800–62.700	20	59.350	53.000–62.875
Tc lymphocytes	17	40.000	34.700–50.600	20	33.200^*	27.150–37.425
Th/Tc lymphocytes	17	1.301	0.923–1.818	20	1.844^*	1.429–2.232
CD4−CD8− T lymphocytes	17	4.200	3.120–6.800	20	7.685^**	5.905–9.140
HLA-DR+ T lymphocytes	17	16.400	10.400–22.900	20	13.250	8.188–22.100
Th lymphocytes
Th1-like lymphocytes	7	21.300	10.095–23.850	20	14.900	12.925–17.375
Th2-like lymphocytes	7	5.290	2.300–26.000	20	3.235	2.745–4.253
Th1/Th2-like lymphocytes	7	1.546	0.841–6.619	20	5.251	3.200–5.975
Th1Th17-like lymphocytes	7	6.920	4.520–8.905	20	8.540	7.108–11.600
Th17-like lymphocytes	7	5.060	3.885–6.060	20	3.960	2.538–5.100
aTreg lymphocytes	11	2.180	1.280–2.650	20	1.860	1.695–2.483
rTreg lymphocytes	11	1.710	0.930–2.930	20	1.240	1.025–2.140
Treg lymphocytes	11	4.210	2.850–5.560	20	3.685	2.981–4.350
non-Treg lymphocytes	11	4.760	4.370–6.720	20	2.835^***	2.265–3.385
FOXP3+ Th lymphocytes	11	8.060	6.440–12.490	20	6.250^*	5.724–7.255
Tcon lymphocytes	11	90.040	84.745–92.140	20	94.430^****	94.118–94.668
Treg/Tcon lymphocytes	11	0.040	0.026–0.059	20	0.039	0.031–0.047
FOXP3+ Th lymphocytes
CD25− lymphocytes	11	36.900	31.950–44.250	20	14.900^***	13.775–23.025
aTreg lymphocytes	11	20.350	16.692–22.094	20	32.796^**	25.258–36.362
rTreg lymphocytes	11	23.932	15.123–28.478	20	20.839	17.529–31.477
non-Treg lymphocytes	11	56.938	52.566–70.045	20	43.09^**	37.758–51.218
Each FOXP3+ Th subset
CD25− cells in aTreg subset	11	28.200	12.850–34.250	20	9.155^***	6.620–13.275
CD25− cells in rTreg subset	11	33.200	14.500–50.600	20	18.300	11.975–42.150
CD25− cells in non-Treg subset	11	51.300	33.600–54.750	20	26.050	23.900–40.125
Tcon lymphocytes
CD25+ cells	11	18.600	13.150–22.250	20	14.550	11.075–18.525
Analysis of STAT
STAT1 (ΔMFI)	15	1068.0	657.5–1744.5	19	145.0^****	111.0–160.5
pSTAT1 (ΔMFI)	9	220.4	44.0–390.0	6	5.0^***	2.5–8.4
STAT5 (ΔMFI)	9	112.0	39.0–209.0	12	13.0^***	6.0–19.0
pSTAT5 (%)	14	13.03	7.735–54.86	16	7.854	6.775–16.99
pSTAT5 (ΔMFI)	15	198.0	126.5–339.0	20	176.0	103.5–210.8

↵* p < 0.05.
↵** p < 0.01.
↵*** p < 0.001.
↵**** p < 0.0001. cSLE: childhood-onset systemic lupus erythematosus; HC: healthy controls; Tc: cytotoxic T lymphocytes; Treg: regulatory Th; rTreg: resting Treg; aTreg: activated Treg; Tcon: conventional T lymphocytes; non-Treg: CD45RA-FOXP3^low activated effector T lymphocytes; pSTAT: phosphorylated STAT; MFI: median fluorescent intensity.