Predominant TRBV usage of activated (CD69+) CD8+ T cells from patients with knee OA, from peripheral blood and IPFP.
| Donor # | PB | IPFP |
|---|---|---|
| 1 | TRBV15 (55)* | TRBV15 (26) |
| TRBV20-1 (17) | TRBV27 (21) | |
| TRBV4-2 (6)** | TRBV29-1 (15) | |
| TRBV24-1 (6)** | TRBV2 (11) | |
| TRBV13 (11) | ||
| 2 | TRBV28 (22) | TRBV15 (16) |
| TRBV4-1 (13) | TRBV19 (14) | |
| TRBV20-1 (9)*** | TRBV20-1 (12) | |
| 3 | TRBV6-4 (17) | TRBV20-1 (27) |
| TRBV20-1 (17) | TRBV4-1 (13) | |
| TRBV15 (17) | TRBV19 (13) | |
| TRBV28 (11) | TRBV29-1 (7)*** | |
| TRBV2 (11) |
Data in parentheses are percentages. Activated CD8+ T cells from peripheral blood (PB) and IPFP of 3 knee OA patients were analyzed for the TCR repertoire by determining their TRBV usage. Shown in this table are the predominant TRBV usages (> 10%) of each donor and from each tissue. Percentages represent the proportion of each TRBV usage from all clones. Number of clones: Donor 1, PB n = 23, IPFP n = 59; Donor 2, PB n = 49, IPFP n = 47; Donor 3, PB n = 21, IPFP n = 16.
↵* This TRBV15 usage by Donor 1 contained clones that were identical (same CDR3β) in 25/27 clones.
↵** TRBV usage of subsequent hierarchy in PB of Donor 1 was included to compensate for the biased large proportion of TRBV15 usage.
↵*** Clones that were < 10% in proportion included to illustrate the overall view of TRBV usage among donors. Values in bold face represent TRBV usages that are overlapping among donors. TRBV: T cell receptor β-variable gene chain; OA: osteoarthritis; IPFP: infrapatellar fat pads; TCR: T cell receptor.