Economic and time comparison of the HLA-B locus PCR-SSO test with the HLA-B*27 targeted tag-SNP assay (rs116488202).
| Variables | HLA-B Locus PCR-SSO | HLA-B*27 Tag-SNP | |
|---|---|---|---|
| Cost per sample, $ | 64.91 | 4.61 | 3.28 |
| Cost per run, $ | 714.00 | 92.16 | 190.02 |
| Hands-on time per run, min | 209 | 151 | 403 |
| Hands-on time per sample, min | 19 | 6.30 | 6.30 |
| Samples tested per run, n | 11† | 20 | 58 |
↵† The HLA-B locus kit is sufficient to perform 16 reactions (11 samples, 4 rotating positive controls, 1 non-template control). Given that it is easy to mix up sample order during the wash cycle step for the PCR-SSO kit, a minimum of 4 controls is strongly recommended. Hands-on time represents the total time for run preparation paperwork, wet work, quality control, result analysis, interpretation, second checking, and reporting. PCR-SSO: PCR-sequence–specific oligonucleotide; SNP: single-nucleotide polymorphism.