Primer sequences (5′–3′) and probes used for amplification of SNP regions in 5 human genes that encode tubular urate transporters.
| SNP | Gene | Localization/reference | Amplicon Size | Primers | Probes |
|---|---|---|---|---|---|
| rs11231825 | SLC22A12/URAT1 | Chromosome 11 | 218 | CCCTAGAGGTCACCAGACCA | CGTGTGTGACTCTCACGCTCT–FL |
| NM_144585.2 | GGGGGTACTCACCTGTCTGA | LC640-AAGCCCATGGCCCAGTCCA–PH | |||
| rs16890979 | SLC2A9/GLUT 9 | Chromosome 4 | 182 | GACAATCACGGTGACCAC | CGGCTCTCAGCCAGGACCTCCTCT–FL |
| NM_001001290.1 | GCTGAAAGTCCATGTTGTC | L670-CCTCTTGGGAAACGTCTGC-PH | |||
| rs17300741 | SLC22A11/OAT4 | Chromosome 11 | 277 | CCAAGATCGCACCACTACA | TGGTGAAGCAGCCAACTC–FL |
| NM_018484.2 | AGAATAAAGCTGAGTCCCAATG | LC640–AGGGACACCCATGCCCAGG-PH | |||
| rs1165196 | SLC17A1/NPT1 | Chromosome 6 | 234 | TTAATGAATTTCTCTCCAGGTCAG | AAAACGTAAAACTACCAATGGAAAT–FL |
| NM_005074.3 | AATGTACACAGAGTCTTTCGTGA | LC640–CCCAGACTGGAAGCGACTTAAGT–PH | |||
| rs2231142 | BCRP/ABCG2 | Chromosome 4 | 128 | GTCTCATTAAAATGCTATTTGCCT | GCTGAGAACTTTAAGTTTTCTCTCACC–FL |
| NM_00482.2 | GAATGACCCTGTTAATCCG | LC610–TCAGAGTGCCCATCACAACATCATCCT–PH |
SNP: single-nucleotide polymorphism.