Expression of genes involved in the transport, metabolism, and mechanism of action of methotrexate (MTX) in rheumatoid synovium. All data are mean gene expression (ng/ml; normalized to GAPDH) ± SEM for all 20 patients or for subgroups receiving or not receiving MTX.
| Synovial Gene Expression | ||||
|---|---|---|---|---|
| Gene | Sample Group, n = 20 | No MTX, n = 9 | MTX, n = 11 | p |
| MTRR | 4.21 ± 0.73 | 2.66 ± 0.60 | 5.48 ± 1.13 | 0.04 |
| MTR | 4.06 ± 0.90 | 2.66 ± 0.66 | 2.88 ± 0.49 | 0.59 |
| ABCB1 | 2.78 ± 0.39 | 2.38 ± 0.53 | 5.4 ± 1.48 | 0.15 |
| FPGS | 1.59 ± 0.36 | 1.2 ± 0.59 | 1.89 ± 0.44 | 0.027 |
| ABCC1 | 1.38 ± 0.18 | 0.86 ± 0.18 | 1.81 ± 0.23 | 0.02 |
| ABCG2 (BCRP) | 0.71 ± 0.18 | 0.45 ± 0.15 | 0.93 ± 0.30 | 0.22 |
| GGH | 0.62 ± 0.09 | 0.43 ± 0.09 | 0.79 ± 0.11 | 0.048 |
| TYMS | 0.42 ± 0.06 | 0.31 ± 0.06 | 0.51 ± 0.08 | 0.09 |
| SLC19A1 (RFC1) | 0.37 ± 0.04 | 0.28 ± 0.04 | 0.45 ± 0.05 | 0.037 |
FPGS: folylpolyglutamate synthetase; GGH: γ-glutamyl hydrolase; TYMS: thymidylate synthase.