Primers and PCR conditions.
| Names | Primers | Annealing Temperature,°C | PCR Size, bp |
|---|---|---|---|
| Exon1-2-F | 5′ CAC CCT TGG ACT GAG CTC T3′ | 64 | 1112 |
| Exon1-2-R | 5′ GGC ATG CCT CAG GCT AGA TG3′ | ||
| Exon3-F | 5′ AGC TCT CTG ACC GAA GGC TC3′ | ||
| Exon3-R | 5′ CCT CTT TCA GTG CAA GTG GG3′ | 60 | 536 |
| c.C167T-F | 5′ AGT GGC AGA AAC ACC CAC CTA CC3′ | ||
| c.C167T-R | 5′ AGG TGG ACA CGA TGC GTG CTC C3′ | 60 | 316 |
| c.C364T-F | 5′ GAG TGG CAG AAA CAC CCA CC3′ | ||
| c.C364T-R | 5′ AAC TGG TGT CCT AGC TCA GG3′ | 63 | 443 |