Study results.
| Normal Osteoblasts | OP Osteoblasts | OA Osteoblasts | |
|---|---|---|---|
| DKK-1 (pg/mg proteins) | |||
| Basal | 450.8 ± 99.70 | 791.7 ± 100.08, p < 0.001 | 302.2 ± 131.70, p < 0.05 |
| Vitamin D | 286.8 ± 127.30, p < 0.05 vs basal | 457.3 ± 90.05, p < 0.05 vs basal | 201.8 ± 78.04, p < 0.05 vs basal |
| DKK-2 (ng/mg proteins) | |||
| Basal | 3.52 ± 1.05 | 1.94 ± 1.09, p < 0.05 | 7.85 ± 0.96, p < 0.001 |
| Vitamin D | 4.18 ± 1.43, p < 0.05 vs basal | 2.89 ± 0.93, p < 0.05 vs basal | 9.62 ± 0.32, p < 0.05 vs basal |
| β-catenin (OD) | |||
| Basal | 0.94 ± 0.06 | 0.645 ± 0.04, p < 0.01 | 1.65 ± 0.09, p < 0.05 |
| Vitamin D | 1.10 ± 0.09 | 0.732 ± 0.07 | 1.59 ± 0.04 |
| RANKL/OPG | |||
| Basal | 0.0124 ± 0.0026 | 0.0241 ± 0.0054, p < 0.001 | 0.0047 ± 0.0017, p < 0.05 |
| Vitamin D | 0.0174 ± 0.0028, p < 0.05 vs basal | 0.0281 ± 0.0056, p < 0.05 vs basal | 0.0056 ± 0.0015, p < 0.05 vs basal |
| Osteocalcin (ng/mg proteins) | |||
| Basal | 19.75 ± 4.02 | 4.17 ± 0.78, p < 0.05 | 56.78 ± 6.7, p < 0.05 |
| Vitamin D | 32.52 ± 6.58, p < 0.05 vs basal | 7.15 ± 1.18, p < 0.05 vs basal | 97.22 ± 3.6, p < 0.05 vs basal |
| ALP (ng/mg proteins) | |||
| Basal | 29.85 ± 4.93 | 12.98 ± 3.82, p < 0.01 | 56.82 ± 9.43, p < 0.05 |
| Vitamin D | 39.25 ± 12.35, p < 0.05 vs basal | 26.36 ± 6.98, p < 0.05 vs basal | 68.02 ± 11.702, p < 0.05 vs basal |
OA: osteoarthritic; OP: osteoporotic; DKK: Dickkopf protein factor; OD: optical density; RANKL/OPG: receptor activator of nuclear factor-κB ligand/osteoprotegerin; ALP: alkaline phosphatase.