Chemicals with biologically meaningful effective concentrations. All compounds satisfy these criteria for being potentially bioactive and experimentally reproducible: R2 > 90%, IC50/EC50 < 10 μM, the upper boundary of 95% CI < 50 μM, and the dose response curves are sigmoidal; R values are derived from dose-response curves.
| Chemical | IC50 (μM) | 95% CI | R2 | Maximum Inhibition, % |
| Celastrol | 2.49 | 0.78–4.20 | 0.94 | 94 |
| Pristimerin | 3.25 | 2.96–3.55 | 1.00 | 93 |
| EC50 (μM) | 95% CI | R2 | Maximum Activation, % | |
| Apigenin | 0.63 | −0.60–1.86 | 0.96 | 193 |
| Biochanin A | 1.33 | 0.81–1.86 | 0.99 | 259 |
| Biochanin A diacetate | 2.31 | 1.50–3.12 | 0.99 | 246 |
| Chrysin | 1.46 | 0.86–2.06 | 0.97 | 244 |
| 6,3′-dimethoxyflavone | 0.09 | 0.01–0.17 | 0.99 | 268 |
| 6,4′-dimethoxyflavone | 0.09 | 0.01–0.17 | 0.99 | 265 |