Antibodies used in this study.
| Specificity | Clone | Antigen Retrieval | Source |
|---|---|---|---|
| Flow cytometry | |||
| RPE-CD29 | MAR4 | BD Pharmingen | |
| RPE-CD34 | 581 | BD Pharmingen | |
| FITC-CD44 | IM7 | BD Pharmingen | |
| FITC-CD45 | HI30 | BD Pharmingen | |
| PE-CD73 | AD2 | BD Pharmingen | |
| PE-Cy5-CD90 | 5E10 | BD Pharmingen | |
| FITC-CD105 | 8E11 | Chemicon | |
| APC-CD117 | YB5.B8 | BD Pharmingen | |
| RPE-CD166 | 3A6 | BD Pharmingen | |
| STRO-1 | NS1-Ag4-1 | Developmental Studies Hybridoma Bank | |
| Immunohistochemistry | |||
| Collagen type I | COL-1 | Hyaluronidase | Abcam |
| Collagen type II | COL-2 | Hyaluronidase | Abcam |
| Chondroitin 4 sulfate | 2B6 | Chondroitinase | ICN Biomedicals, Inc. |
| Integrin subunit ß1 | 4B7R | Abcam | |
| Keratan sulfate | 5D4 | Chondroitinase | Seikagaku America |
| CD90 | 5E10 | BD Pharmingen | |
| Immunofluorescence | |||
| Mouse anti-human CD44 HCAM | DF1485 | Santa Cruz Biotechnology | |
| Mouse anti-human CD90 (Thy-1) | BD Pharmingen | ||
| Mouse anti-human CD105-FITC | MCA1557F | Serotec | |
| Rabbit anti-mouse immunoglobulin-FITC | Dako | ||
| Goat anti-mouse immunoglobulin-RPE | Dako |
RPE: R-phycoerythrin; FITC: fluorescein; PE: phycoerythrin; APC: allophycocyanin; HCAM: homing cell adhesion molecule.