SNP Detected | Type | Primer Sequence | Product Size, bp | RFLP Enzyme |
---|---|---|---|---|
rs26653 | Nonsynonymous | F: 5′ ACT CTG TGA CCG TGT AGT GA3′ R: 5′AAC AGC CCT AGG AAA CTA A33′ | 1213 | Bsu36I |
rs27434 | Synonymous | F: 5′ GAG TGG GAC CTT GTT TCC AA3′ R: 5′ TCA GTT CAG GAT GGG TCA CA3′ | 813 | HphI |
rs27640 | Intronic | F: 5′ CTT GTT GTG TTG CTG TTT GG3′ R: 5′ GCA GAG TTT CTG TAA GCC ATGA3′ | 683 | BsmAI |
rs27529 | Synonymous | F: 5′ TGA TGC TAA TTT GCC CCA TC3′ R: 5′ CAG TGA GAA GCA TTA CCA AGGA3′ | 841 | HhaI |
rs26510 | Intronic | F: 5′ TGA TGC TAA TTT GCC CCA TC3′ R: 5′ CAG TGA GAA GCA TTA CCA AGGA3′ | 841 | MboI |
rs27582 | 3′ UTR | F: 5′ CTG GTG TGA ATT GGG AAG AA3′ F: 5′ GCA ACA TTT GAG AGG GCA GT3′ | 980 | BsmAI |
PCR-RFLP: polymerase chain reaction-restriction fragment-length polymorphism; SNP: single-nucleotide polymorphism; bp: base pairs.