Table 1.

PCR-RFLP information for the 6 candidate SNP selected by sequencing.

SNP DetectedTypePrimer SequenceProduct Size, bpRFLP Enzyme
rs26653NonsynonymousF: 5′ ACT CTG TGA CCG TGT AGT GA3′
R: 5′AAC AGC CCT AGG AAA CTA A33′
1213Bsu36I
rs27434SynonymousF: 5′ GAG TGG GAC CTT GTT TCC AA3′
R: 5′ TCA GTT CAG GAT GGG TCA CA3′
813HphI
rs27640IntronicF: 5′ CTT GTT GTG TTG CTG TTT GG3′
R: 5′ GCA GAG TTT CTG TAA GCC ATGA3′
683BsmAI
rs27529SynonymousF: 5′ TGA TGC TAA TTT GCC CCA TC3′
R: 5′ CAG TGA GAA GCA TTA CCA AGGA3′
841HhaI
rs26510IntronicF: 5′ TGA TGC TAA TTT GCC CCA TC3′
R: 5′ CAG TGA GAA GCA TTA CCA AGGA3′
841MboI
rs275823′ UTRF: 5′ CTG GTG TGA ATT GGG AAG AA3′
F: 5′ GCA ACA TTT GAG AGG GCA GT3′
980BsmAI
  • PCR-RFLP: polymerase chain reaction-restriction fragment-length polymorphism; SNP: single-nucleotide polymorphism; bp: base pairs.