Table 1.

Details of quantitative PCR.

GeneAccession No.Primer and ProbeAmplicon LengthPCR EfficiencyLower Limit of Detection (molecules)
Toll-like receptor 4NM_138554500 (+) GAG CCT TTT CTG GAC TAT CAA G*
582 (−) TCC AAT GGG GAA GTT CTC TAG*
554 (−) FAM-AGA TTT GTC TCC ACA GCC ACC AGC-BHQ1†		81 bases95%180
Toll-like receptor 5NM_0032682209 (+) GCC ATC TGA CTG CAT TAA GG*
2284 (−) GCA GGT AAA TCA TTG TGA GAA AG*
2237 (+) FAM-CCT CAA CTC CAA CAG GCT GAC AGT-BHQ1†		84 bases95%170

  • ↵* Primer sequence.

  • ↵† Probe sequence.