Oligonucleotides used for genotyping of SNP rs10865331 and rs2242944 and for PCR detection of HLA-B27 and exon 8 of p53. The annealing temperature of the PCR was 62°C for HLA-B27 and p53 detection, and 57°C for rs10865331 and rs2242944 genotyping.
| Forward | Reverse | |
|---|---|---|
| rs10865331 (2p15) | CTG ACT TTG GTG CCG TAT CTA CCA A TACT TTG GTG CCG TAT CTA CCA G | TGA GGC AAT GGC CAC TTT ACG ACT T |
| rs2242944 (21q22) | TGA TAA TTC TGT ATG TTT TAG TAT CAC TGG T ATAAT TCT GTA TGT TTT AGT ATC ACT GGC | GAG GTG GGG AGA GTT CCA AAG G |
| HLA-B27 p53 | GCT ACG TGG ACG ACA CGC T TAG GAC CTG ATT TCC TTA CTG CCT C | CAG TCT GTG CCT TGG CCT TGC AAC TGC ACC CTT GGT CTC CTC CAC C |