Table 3.

OMERACT 9 Soluble Biomarker Working Group minimum standards for the handling and processing of biomarker samples.

Type of sampleSerum, urineStandardized sample collection (e.g., tubes)
Fasting sample
Time of collection2–4 hours after risingDiurnal variation is common with musculoskeletal biomarkers22
Time of sample processingWithin 2 hours of collectionStandardized centrifugation procedure. Keep sample at 4°C prior to centrifugation23
Storage of samplesSample storage in 300–400 μl aliquots in −70°C freezerAvoid use of sample after 3 freeze/thaw cycles
Sample transportExpress delivery on dry iceUse ample dry ice