Recommendation | Comment | |
---|---|---|
Type of sample | Serum, urine | Standardized sample collection (e.g., tubes) |
Fasting sample | ||
Time of collection | 2–4 hours after rising | Diurnal variation is common with musculoskeletal biomarkers22 |
Time of sample processing | Within 2 hours of collection | Standardized centrifugation procedure. Keep sample at 4°C prior to centrifugation23 |
Storage of samples | Sample storage in 300–400 μl aliquots in −70°C freezer | Avoid use of sample after 3 freeze/thaw cycles |
Sample transport | Express delivery on dry ice | Use ample dry ice |