Significant changes in markers expressed in various ANKH-transfectants relative to that of the neo controls at each time point.
| Transfectant (ITS) | Sox9 | Sox5 | Sox6 | Col-2αl | Col X | BSP | MMP-13 | ENPP-1 | ALP Activity | TNAP Protein |
|---|---|---|---|---|---|---|---|---|---|---|
| ANKH-wt | ||||||||||
| Day 0 | ↑1.4× | ↑7.9× | ↓5× | ↑7.7× | ND | |||||
| Day 10 | ↓1.7× | ↓2× | ↓3× | ↑2× | ↓2.5× | ND | ||||
| Day 17 | ↓3× | ↓2.5× | ↓3× | ↓3× | ||||||
| Day 23 | ↑1.6× | ↓2× | ↓10× | ↓5× | ↓2.5× | ND | ↓2× | ↓2× | ||
| ANKH-M48T | ||||||||||
| Day 0 | ↑2× | ↓3.3× | ↑2× | ND | ↑2× | |||||
| Day 10 | ↑8.6× | ↓10× | ND | ↑2× | ↑2× | |||||
| Day 17 | ↑2× | ↑1.5× | ↑1.7× | ↓3× | ↑2× | ↓2.5× | ↑2× | ↑2× | ↑2× | |
| Day 23 | ↑1.6× | ↑1.3× | ↓3× | ↓5× | ↓10× | ↑2× | ND | |||
ANKH: human homolog of progressive ankylosis; Col: collagen; BSP: bone sialoprotein; MMP: matrix metalloproteinase; ND: not done.