RT Journal Article
SR Electronic
T1 Cytotoxicity responses to peptide antigens in rheumatoid arthritis and ankylosing spondylitis.
JF The Journal of Rheumatology
JO J Rheumatol
FD The Journal of Rheumatology
SP 972
OP 978
VO 30
IS 5
A1 Clyde Wilson
A1 Taha Rashid
A1 Harmale Tiwana
A1 Huryia Beyan
A1 Lucy Hughes
A1 Sukvinder Bansal
A1 Alan Ebringer
A1 Allen Binder
YR 2003
UL http://www.jrheum.org/content/30/5/972.abstract
AB OBJECTIVE: To measure levels of IgG antibodies against structurally related synthetic peptides of HLA-DRB1*0404, type XI collagen, and Proteus mirabilis in patients with rheumatoid arthritis (RA) and HLA-B*2705 and Klebsiella pneumoniae in patients with ankylosing spondylitis (AS), and to determine whether sera from RA and AS patients are cytotoxic for sheep red blood cells (SRBC) coated with HLA-DRB1*0404, type XI collagen, or HLA-B*2705. METHODS: Sera from 51 patients with RA, 34 with AS, and 38 healthy controls were tested against synthetic EQRRAA, ESRRAL, LRREI, and IRRET peptides by ELISA. Sera from patients and controls were also tested for reactivity in complement mediated cytotoxicity with SRBC coated with EQRRAA and HLA-B*2705, LRREI peptides. RESULTS: Antibodies to synthetic peptides containing EQRRAA, ESRRAL, LRREI, and IRRET were significantly increased in RA patients compared with AS patients (p < 0.001) and controls (p < 0.001). The percentage lysis data for SRBC coated with EQRRAA and LRREI peptides were significantly higher for RA sera (p < 0.001) compared to control sera. Percentage lysis for SRBC coated with HLA-B*2705 peptide was significantly higher for AS sera (p < 0.001) compared to control sera. CONCLUSION: Our results suggest that antibodies against antigenic determinants of P. mirabilis in RA and K. pneumoniae in AS have cytotoxic properties on structurally related host proteins. These cytotoxic antibodies together with T cell interactions could be relevant in the etiopathogenesis of RA and AS.