PT  - JOURNAL ARTICLE
AU  - Sachiko Iwaki-Egawa
AU  - Hiroaki Matsuno
AU  - Kazuo Yudoh
AU  - Fujio Nakazawa
AU  - Kyousuke Miyazaki
AU  - Akira Ochiai
AU  - Shunsei Hirohata
AU  - Masatoshi Shimizu
AU  - Yasuhiro Watanabe
TI  - High diagnostic value of anticalpastatin autoantibodies in rheumatoid arthritis detected by ELISA using human erythrocyte calpastatin as antigen.
DP  - 2004 Jan 01
TA  - The Journal of Rheumatology
PG  - 17--22
VI  - 31
IP  - 1
4099  - http://www.jrheum.org/content/31/1/17.short
4100  - http://www.jrheum.org/content/31/1/17.full
SO  - J Rheumatol2004 Jan 01; 31
AB  - OBJECTIVE: To develop a quantitative method of measuring autoantibodies against human calpastatin in rheumatoid arthritis (RA) and to determine their diagnostic value compared with other autoimmune and articular diseases. METHODS: We performed a highly sensitive ELISA for IgG and IgM anticalpastatin autoantibodies in human sera using human erythrocyte calpastatin as an antigen. Samples were diluted 1:2000 for the measurement of IgG and 1:400 for IgM. RESULTS: IgG anticalpastatin antibodies were found in the sera of 48 of 58 patients (82.8%) with RA. In contrast, IgG anticalpastatin antibodies were found in the sera of only 2 of 11 (8.3%) patients with osteoarthritis (OA). Compared to sera from patients with other autoimmune diseases, anticalpastatin antibody sensitivity for RA was better than that of systemic lupus erythematosus (5.6%), systemic sclerosis (0%), mixed connective tissue disease (0%), and Sjögren's syndrome (20%). IgG anticalpastatin antibodies also showed high specificity (96.1%) for RA. Almost 90% of patients with RA were positive for IgG or IgM anticalpastatin antibodies. CONCLUSION: We have developed a simple, sensitive, specific, and quantitative ELISA for anticalpastatin antibodies that may have a high diagnostic value for RA.