RT Journal Article
SR Electronic
T1 Imaging skeletal pathology in mutant mice by microcomputed tomography.
JF The Journal of Rheumatology
JO J Rheumatol
FD The Journal of Rheumatology
SP 2659
OP 2665
VO 30
IS 12
A1 Alice F Ford-Hutchinson
A1 David M L Cooper
A1 Benedikt Hallgrímsson
A1 Frank R Jirik
YR 2003
UL http://www.jrheum.org/content/30/12/2659.abstract
AB OBJECTIVE: We describe the utility of microcomputed tomography ( micro CT) for imaging skeletal abnormalities in rodent model systems. For the purpose of illustration, the progressive ankylosis (ank) mutant was selected. ank mice develop prominent articular and periarticular calcifications at multiple anatomical sites, including paws, elbows, knees, and vertebrae. METHODS: Forelimbs, hindlimbs, and proximal tail vertebrae of 4-month-old female ank/ank mice were scanned at 15 micro m resolution using a SkyScan 1072 micro CT instrument and images were generated using Analyze 4.0 software. RESULTS: This technique was able to show, in 3-dimensional images, the abnormal calcification of ank/ank mice, which was readily observed within joint surfaces, on periosteal surfaces, sesamoid bones, menisci, and joint capsules, as well as other periarticular ligamentous structures. CONCLUSION: As illustrated by the example of the progressive ankylosis mutant, micro CT represents a powerful emerging tool for identifying and monitoring the progression of developmental or acquired skeletal abnormalities within rodent models.