PT  - JOURNAL ARTICLE
AU  - Isabel Ferreirós-Vidal
AU  - Juan Amarelo
AU  - Francisco Barros
AU  - Angel Carracedo
AU  - Juan J Gómez-Reino
AU  - Antonio Gonzalez
TI  - Lack of association of ankylosing spondylitis with the most common NOD2 susceptibility alleles to Crohn's disease.
DP  - 2003 Jan 01
TA  - The Journal of Rheumatology
PG  - 102--104
VI  - 30
IP  - 1
4099  - http://www.jrheum.org/content/30/1/102.short
4100  - http://www.jrheum.org/content/30/1/102.full
SO  - J Rheumatol2003 Jan 01; 30
AB  - OBJECTIVE: To investigate whether the 3 most common mutations in the NOD2 gene that confer susceptibility to Crohn's disease (CD) are also associated with ankylosing spondylitis (AS). METHODS: DNA from 112 patients with AS and 168 controls of homogenous Spanish ancestry were studied. The frequencies of the pathogenic alleles of NOD2 (3020insC, 2722G>C, and 2104C>T) were determined by analysis of the melting curves after hybridization with FRET probes on a Light Cycler real-time polymerase chain reaction (PCR) system. Results. NOD2 allelic frequencies in controls (3020insC, 0.009; 2722G>C, 0.009; 2104C>T, 0.042) did not significantly differ from patients with AS (3020insC, 0.009; 2722G>C, 0.004; 2104C>T, 0.031). CONCLUSION: The 3 most common CD NOD2 mutations do not contribute to disease susceptibility to AS, and therefore do not explain the susceptibility locus for AS in chromosome 16q.