PT  - JOURNAL ARTICLE
AU  - Mercado Monica Vazquez-Del
AU  - Araceli Garcia-Gonzalez
AU  - José Francisco Muñoz-Valle
AU  - Trinidad Garcia-Iglesias
AU  - Gloria Martinez-Bonilla
AU  - Guislaine Bernard-Medina
AU  - Adriana Sanchez-Ortiz
AU  - José M Ornelas-Aguirre
AU  - Mario Salazar-Paramo
AU  - Jorge I Gamez-Nava
AU  - Laura Gonzalez-Lopez
TI  - Interleukin 1beta (IL-1beta), IL-10, tumor necrosis factor-alpha, and cellular proliferation index in peripheral blood mononuclear cells in patients with ankylosing spondylitis.
DP  - 2002 Mar 01
TA  - The Journal of Rheumatology
PG  - 522--526
VI  - 29
IP  - 3
4099  - http://www.jrheum.org/content/29/3/522.short
4100  - http://www.jrheum.org/content/29/3/522.full
SO  - J Rheumatol2002 Mar 01; 29
AB  - OBJECTIVE: To evaluate cytokine production and cellular proliferation index (CPI) in peripheral blood mononuclear cells (PBMC) of patients with ankylosing spondylitis (AS), and their association with clinical variables. METHODS: In a cross sectional study we compared the production of tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), and IL-10 and CPI in response to phytohemagglutinin (PHA) in PBMC of 27 patients with AS and 24 healthy controls. We also assessed clinical characteristics including disease activity index (BASDAI) and functional index (BASFI). RESULTS: Levels of IL-1beta were higher in patients with AS (median 242 pg/ml) than in controls (median 65 pg/ml); p = 0.002. No differences were observed in median levels of TNF-alpha or IL-10 between AS and controls. Patients had a reduction in CPI (1.2 in AS vs 1.8 in controls; p < 0.001). A positive correlation was observed between IL-10 production and age (rho = 0.34, p = 0.01). A borderline negative correlation was observed between CPI and age (rho = -0.26, p = 0.07). CONCLUSION: Patients with AS had high production of IL-1beta compared with controls and a poor response in CPI. These findings may explain the lack of response for microbial antigens mediated by the innate immune response.