RT Journal Article
SR Electronic
T1 Differential expression patterns of secreted frizzled related protein genes in synovial cells from patients with arthritis.
JF The Journal of Rheumatology
JO J Rheumatol
FD The Journal of Rheumatology
SP 2266
OP 2270
VO 29
IS 11
A1 Kosei Ijiri
A1 Ryusaku Nagayoshi
A1 Noriko Matsushita
A1 Hiromichi Tsuruga
A1 Noboru Taniguchi
A1 Akira Gushi
A1 Harutoshi Sakakima
A1 Setsuro Komiya
A1 Takami Matsuyama
YR 2002
UL http://www.jrheum.org/content/29/11/2266.abstract
AB OBJECTIVE: To detect expression of the secreted frizzled related protein (sFRP) gene in synovial cells from patients with arthritis. METHODS: Expression of sFRP-1, 2, 3, 4, and 5 genes was detected in synovial cells from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. To identify synovial cell populations expressing sFRP-1, 3, and 4 genes, expression was compared in macrophage-rich populations and fibroblast-like cell-rich populations by RT-PCR. Levels of expression of these genes were also studied in activated peripheral blood mononuclear cells (PBMC) and activated skin fibroblasts. RESULTS: Expression of the sFRP-1, 3, and 4 genes was observed in both RA and OA synovial cells. sFRP-1 and 4 genes were expressed predominantly in fibroblast-like cell-rich populations, and the sFRP-3 gene was expressed predominantly in macrophage-rich populations. Levels of expression of sFRP-3 and 4 genes were elevated in activated PBMC and activated skin fibroblasts. CONCLUSION: Our findings suggest that sFRP-1, 3, and 4 may play different roles in the pathogenesis of synovitis.