PT - JOURNAL ARTICLE AU - Kosei Ijiri AU - Ryusaku Nagayoshi AU - Noriko Matsushita AU - Hiromichi Tsuruga AU - Noboru Taniguchi AU - Akira Gushi AU - Harutoshi Sakakima AU - Setsuro Komiya AU - Takami Matsuyama TI - Differential expression patterns of secreted frizzled related protein genes in synovial cells from patients with arthritis. DP - 2002 Nov 01 TA - The Journal of Rheumatology PG - 2266--2270 VI - 29 IP - 11 4099 - http://www.jrheum.org/content/29/11/2266.short 4100 - http://www.jrheum.org/content/29/11/2266.full SO - J Rheumatol2002 Nov 01; 29 AB - OBJECTIVE: To detect expression of the secreted frizzled related protein (sFRP) gene in synovial cells from patients with arthritis. METHODS: Expression of sFRP-1, 2, 3, 4, and 5 genes was detected in synovial cells from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. To identify synovial cell populations expressing sFRP-1, 3, and 4 genes, expression was compared in macrophage-rich populations and fibroblast-like cell-rich populations by RT-PCR. Levels of expression of these genes were also studied in activated peripheral blood mononuclear cells (PBMC) and activated skin fibroblasts. RESULTS: Expression of the sFRP-1, 3, and 4 genes was observed in both RA and OA synovial cells. sFRP-1 and 4 genes were expressed predominantly in fibroblast-like cell-rich populations, and the sFRP-3 gene was expressed predominantly in macrophage-rich populations. Levels of expression of sFRP-3 and 4 genes were elevated in activated PBMC and activated skin fibroblasts. CONCLUSION: Our findings suggest that sFRP-1, 3, and 4 may play different roles in the pathogenesis of synovitis.