%0 Journal Article %A Naomi Kobayashi %A Tomihisa Koshino %A Masaaki Uesugi %A Naoki Yokoo %A Ke-Qin Xin %A Kenji Okuda %A Hiroaki Mizukami %A Keiya Ozawa %A Tomoyuki Saito %T Gene marking in adeno-associated virus vector infected periosteum derived cells for cartilage repair. %D 2002 %J The Journal of Rheumatology %P 2176-2180 %V 29 %N 10 %X OBJECTIVE: To evaluate both the potential for transferring genes to periosteal cells using an adeno-associated virus (AAV) vector and the potential for gene expression after transplantation of those cells to a cartilage defect in vivo. METHODS: Periosteum was obtained from the tibia of 6-week-old rabbits and enzymatically digested. The isolated periosteum derived cells were cultured and the subconfluence cells were infected with a recombinant AAV expressing the LacZ gene (AAV-LacZ). Collagen gel containing the LacZ transferred, periosteum derived cells was transplanted into a full thickness articular cartilage defect in 10 rabbits. RESULTS: Infected cells still growing on the plate continued to express LacZ at least 12 weeks after AAV infection, with the highest percentage of LacZ positive cells reaching 74.4%. The LacZ positive cells were recognized at the transplant sites in 8 out of 10 knees. CONCLUSION: Gene expression in periosteum derived cells was sustained in vitro for at least 12 weeks using the AAV vector, and for 2 weeks ex vivo after transplantation into a cartilage defect. %U https://www.jrheum.org/content/jrheum/29/10/2176.full.pdf