TY - JOUR T1 - Gene marking in adeno-associated virus vector infected periosteum derived cells for cartilage repair. JF - The Journal of Rheumatology JO - J Rheumatol SP - 2176 LP - 2180 VL - 29 IS - 10 AU - Naomi Kobayashi AU - Tomihisa Koshino AU - Masaaki Uesugi AU - Naoki Yokoo AU - Ke-Qin Xin AU - Kenji Okuda AU - Hiroaki Mizukami AU - Keiya Ozawa AU - Tomoyuki Saito Y1 - 2002/10/01 UR - http://www.jrheum.org/content/29/10/2176.abstract N2 - OBJECTIVE: To evaluate both the potential for transferring genes to periosteal cells using an adeno-associated virus (AAV) vector and the potential for gene expression after transplantation of those cells to a cartilage defect in vivo. METHODS: Periosteum was obtained from the tibia of 6-week-old rabbits and enzymatically digested. The isolated periosteum derived cells were cultured and the subconfluence cells were infected with a recombinant AAV expressing the LacZ gene (AAV-LacZ). Collagen gel containing the LacZ transferred, periosteum derived cells was transplanted into a full thickness articular cartilage defect in 10 rabbits. RESULTS: Infected cells still growing on the plate continued to express LacZ at least 12 weeks after AAV infection, with the highest percentage of LacZ positive cells reaching 74.4%. The LacZ positive cells were recognized at the transplant sites in 8 out of 10 knees. CONCLUSION: Gene expression in periosteum derived cells was sustained in vitro for at least 12 weeks using the AAV vector, and for 2 weeks ex vivo after transplantation into a cartilage defect. ER -