TY - JOUR T1 - Decreased arachidonic acid release in peripheral blood monocytes of patients with systemic lupus erythematosus. JF - The Journal of Rheumatology JO - J Rheumatol SP - 2012 LP - 2017 VL - 28 IS - 9 AU - S Sipka AU - S Szántó AU - K Szucs AU - I Kovács AU - E Kiss AU - P Antal-Szamás AU - G Lakos AU - M Aleksza AU - A Illés AU - P Gergely AU - G Szegedi Y1 - 2001/09/01 UR - http://www.jrheum.org/content/28/9/2012.abstract N2 - OBJECTIVE: To investigate the release of arachidonic acid (AA) in unfractionated peripheral blood mononuclear cells (PBMC), separated monocytes and T lymphocytes of patients with systemic lupus erythematosus (SLE). METHODS: AA release was measured in cells from 56 patients with SLE and from 48 controls. Of the 56 patients with SLE, 38 were receiving glucocorticosteroids and 18 were not. [3H]AA was incorporated into the membranes of PBMC and purified subsets of monocytes and T lymphocytes. The release of [3H]AA was measured both in nonstimulated cells cultured for 24 h and in cell cultures stimulated by phorbol ester (PMA) and Ca2+ ionophore for 4 h. RESULTS: In the PBMC of SLE patients not taking glucocorticosteroids, the release of AA was decreased in both stimulated and nonstimulated cells. There was a decrease of AA production in monocytes but not in T lymphocytes. This phenomenon could be observed in the active and inactive phases of the disease. CONCLUSION: A defect in AA production may exist in the peripheral monocytes of patients with SLE, resulting in decreased release of AA in patients not receiving glucocorticosteroid therapy. ER -