PT  - JOURNAL ARTICLE
AU  - Bonardelle, D
AU  - Bobé, P
AU  - Reynès, M
AU  - Amouroux, J
AU  - Tricottet, V
AU  - Godeau, F
AU  - Kiger, N
TI  - Inflammatory arthropathy in MRL hematopoietic chimeras undergoing Fas mediated graft-versus-host syndrome.
DP  - 2001 May 01
TA  - The Journal of Rheumatology
PG  - 956--961
VI  - 28
IP  - 5
4099  - http://www.jrheum.org/content/28/5/956.short
4100  - http://www.jrheum.org/content/28/5/956.full
SO  - J Rheumatol2001 May 01; 28
AB  - OBJECTIVE: To determine whether overexpression of the Fas ligand (FasL) on activated lpr T lymphocytes could induce arthritic lesions when grafted into syngeneic wild-type MRL mice expressing normal Fas receptor levels. METHODS: Lethally irradiated MRL+/+ mice were reconstituted with congenic MRL/lpr bone marrow cells and splenocytes overexpressing FasL. Fas-mediated cytotoxic properties of repopulating lpr splenic lymphocytes were evaluated in vitro. Simultaneously, the hind paw ankles of the hematopoietic chimeras were histologically examined. RESULTS: The lpr lymphocytes repopulating the spleen overexpressed FasL and had in vitro Fas-mediated cytotoxic activity. Simultaneously, in vivo, articular (synovitis, pannus) and periarticular (periostitis) inflammation with bone resorption were observed. CONCLUSION: Arthritic lesions may be induced in Fas-expressing recipients by persistent engrafted syngeneic lymphocytes overexpressing FasL.