PT  - JOURNAL ARTICLE
AU  - Sarah M. Wade
AU  - Trudy McGarry
AU  - Siobhan C. Wade
AU  - Ursula Fearon
AU  - Douglas J. Veale
TI  - Serum MicroRNA Signature as a Diagnostic and Therapeutic Marker in Patients with Psoriatic Arthritis
AID  - 10.3899/jrheum.190602
DP  - 2020 Dec 01
TA  - The Journal of Rheumatology
PG  - 1760--1767
VI  - 47
IP  - 12
4099  - http://www.jrheum.org/content/47/12/1760.short
4100  - http://www.jrheum.org/content/47/12/1760.full
SO  - J Rheumatol2020 Dec 01; 47
AB  - Objective MicroRNA (miRNA) are small endogenous regulatory RNA molecules that have emerged as potential therapeutic targets and biomarkers in autoimmunity. Here, we investigated serum miRNA levels in patients with psoriatic arthritis (PsA) and further assessed a serum miRNA signature in therapeutic responder versus nonresponder PsA patients.Methods Serum samples were collected from healthy controls (HC; n = 20) and PsA patients (n = 31), and clinical demographics were obtained. To examine circulatory miRNA in serum from HC and PsA patients, a focused immunology miRNA panel was analyzed utilizing a miRNA Fireplex assay (FirePlex Bioworks Inc.). MiRNA expression was further assessed in responders versus nonresponders according to the European League Against Rheumatism response criteria.Results Six miRNA (miR-221-3p, miR-130a-3p, miR-146a-5p, miR-151-5p, miR-26a-5p, and miR-21-5p) were significantly higher in PsA compared to HC (all P < 0.05), with high specificity and sensitivity determined by receiver-operating characteristic curve analysis. Analysis of responder versus nonresponders demonstrated higher baseline levels of miR-221-3p, miR-130a-3p, miR-146a-5p, miR-151-5p, and miR-26a-5p were associated with therapeutic response.Conclusion This study identified a 6-serum microRNA signature that could be attractive candidates as noninvasive markers for PsA and may help to elucidate the disease pathogenesis.