TY - JOUR T1 - Persistence of Crystals in Stored Synovial Fluid Samples JF - The Journal of Rheumatology JO - J Rheumatol SP - 1416 LP - 1423 DO - 10.3899/jrheum.190468 VL - 47 IS - 9 AU - Sonia Pastor AU - José-Antonio Bernal AU - Rocío Caño AU - Silvia Gómez-Sabater AU - Fernando Borras AU - Mariano Andrés Y1 - 2020/09/01 UR - http://www.jrheum.org/content/47/9/1416.abstract N2 - Objective. Lack of access to polarized light microscopy is often cited as an argument to justify the clinical diagnosis of crystal-related arthritis. We assessed the influence of time since sampling and preservation methods on crystal identification in synovial fluid (SF) samples under polarized light microscopy.Methods. This was a prospective, longitudinal, observational factorial study, analyzing 30 SF samples: 12 with monosodium urate (MSU) crystals and 18 with calcium pyrophosphate (CPP) crystals. Each SF sample was divided into 4 subsamples (120 subsamples in total). Two were stored in each type of preserving agent, heparin or ethylenediamine tetraacetic acid (EDTA), at room temperature or at 4°C. Samples were analyzed the following day (T1), at 3 days (T2), and at 7 days (T3) by simple polarized light microscopy, and the presence of crystals was recorded.Results. The identification of crystals in the MSU group was similar between groups, with crystals observed in 11/12 (91.7%) room temperature samples and in 12/12 (100%) refrigerated samples at T3. Identification of CPP crystals tended to decrease in all conditions, especially when preserved with EDTA at room temperature [12/18 (66.7%) at T3], while less reduction was seen in refrigerated heparin-containing tubes.Conclusion. Preserving samples with heparin in refrigerated conditions allows delayed microscopic examination for crystals. Avoiding crystal-proven diagnosis because of the immediate unavailability of microscopy no longer appears justified. ER -