@article {Gallo1485, author = {Alessia Gallo and Serena Vella and Fabio Tuzzolino and Nicola Cuscino and Antonella Cecchettini and Francesco Ferro and Marta Mosca and Ilias Alevizos and Stefano Bombardieri and Pier Giulio Conaldi and Chiara Baldini}, title = {MicroRNA-mediated Regulation of Mucin-type O-glycosylation Pathway: A Putative Mechanism of Salivary Gland Dysfunction in Sj{\"o}gren Syndrome}, volume = {46}, number = {11}, pages = {1485--1494}, year = {2019}, doi = {10.3899/jrheum.180549}, publisher = {The Journal of Rheumatology}, abstract = {Objective. To investigate microRNA (miRNA) that is potentially implicated in primary Sj{\"o}gren syndrome (pSS){\textendash}related salivary hypofunction in labial salivary glands and to study miRNA-mediated mechanisms underlying oral dryness and altered rheology, focusing on the mucin O-glycosylation pathway.Methods. We performed miRNA expression profiling in minor salivary gland samples of patients with pSS presenting a different impairment in their unstimulated salivary flow rate. A computational in silico analysis was performed to identify genes and pathways that might be modulated by the deregulated miRNA that we had identified. To confirm in silico analysis, expression levels of genes encoding for glycosyltransferases and glycan-processing enzymes were investigated using Human Glycosylation-RT2 Profiler PCR Array.Results. Among 754 miRNA analyzed, we identified 126 miRNA that were significantly deregulated in pSS compared to controls, with a trend that was inversely proportional with the impairment of salivary flow rates. An in silico approach pinpointed that several upregulated miRNA in patients with pSS target important genes in the mucin O-glycosylation. We confirmed this prediction by quantitative real-time PCR, highlighting the downregulation of some glycosyltransferase and glycosidase genes in pSS samples compared to controls, such as GALNT1, responsible for mucin-7 glycosylation.Conclusion. Collectively, our data suggest that the expression of different predicted miRNA-target genes in the mucin type O-glycan biosynthesis pathway is altered in pSS patients with low salivary flow and that the miRNA expression profile could influence the glycosidase expression levels and consequently the rheology in pSS.}, issn = {0315-162X}, URL = {https://www.jrheum.org/content/46/11/1485}, eprint = {https://www.jrheum.org/content/46/11/1485.full.pdf}, journal = {The Journal of Rheumatology} }