TY - JOUR T1 - CXCL10 and TRAIL Are Upregulated by TXNDC5 in Rheumatoid Arthritis Fibroblast-like Synoviocytes JF - The Journal of Rheumatology JO - J Rheumatol SP - 335 LP - 340 DO - 10.3899/jrheum.170170 VL - 45 IS - 3 AU - Bing Xu AU - Jian Li AU - Changsun Wu AU - Chunyan Liu AU - Xinfeng Yan AU - Xiaotian Chang Y1 - 2018/03/01 UR - http://www.jrheum.org/content/45/3/335.abstract N2 - Objective. Thioredoxin domain containing 5 (TXNDC5) is highly expressed in synovial membranes of rheumatoid arthritis (RA). Our study aimed to investigate the pathogenic role of TXNDC5 in RA.Methods. PCR arrays, CCK-8 assays, flow cytometry, and transwell migration assays were used to analyze cultured rheumatoid arthritis synovial fibroblasts (RASF).Results. Increased CXCL10 and tumor necrosis factor-related apoptosis-inducing ligand levels were detected in RASF transfected with anti-TXNDC5 small interfering RNA (siRNA), and decreased expression was detected in RASF transfected with TXNDC5-expressing plasmids. Significantly attenuated RASF proliferation and migration, and increased RASF apoptosis, were observed in the siRNA-transfected RASF.Conclusion. Downregulation of TXNDC5 could contribute to RASF antiangiogenic and proapoptotic features through the suppression of CXCL10 and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). ER -