PT - JOURNAL ARTICLE AU - Bing Xu AU - Jian Li AU - Changsun Wu AU - Chunyan Liu AU - Xinfeng Yan AU - Xiaotian Chang TI - CXCL10 and TRAIL Are Upregulated by TXNDC5 in Rheumatoid Arthritis Fibroblast-like Synoviocytes AID - 10.3899/jrheum.170170 DP - 2018 Mar 01 TA - The Journal of Rheumatology PG - 335--340 VI - 45 IP - 3 4099 - http://www.jrheum.org/content/45/3/335.short 4100 - http://www.jrheum.org/content/45/3/335.full SO - J Rheumatol2018 Mar 01; 45 AB - Objective. Thioredoxin domain containing 5 (TXNDC5) is highly expressed in synovial membranes of rheumatoid arthritis (RA). Our study aimed to investigate the pathogenic role of TXNDC5 in RA.Methods. PCR arrays, CCK-8 assays, flow cytometry, and transwell migration assays were used to analyze cultured rheumatoid arthritis synovial fibroblasts (RASF).Results. Increased CXCL10 and tumor necrosis factor-related apoptosis-inducing ligand levels were detected in RASF transfected with anti-TXNDC5 small interfering RNA (siRNA), and decreased expression was detected in RASF transfected with TXNDC5-expressing plasmids. Significantly attenuated RASF proliferation and migration, and increased RASF apoptosis, were observed in the siRNA-transfected RASF.Conclusion. Downregulation of TXNDC5 could contribute to RASF antiangiogenic and proapoptotic features through the suppression of CXCL10 and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand).