RT Journal Article
SR Electronic
T1 Frequency and Phenotype of T Helper 17 Cells in Peripheral Blood and Synovial Fluid of Patients with Reactive Arthritis
JF The Journal of Rheumatology
JO J Rheumatol
FD The Journal of Rheumatology
SP 2096
OP 2099
DO 10.3899/jrheum.100146
VO 37
IS 10
A1 HUI SHEN
A1 JANE C. GOODALL
A1 J.S. HILL GASTON
YR 2010
UL http://www.jrheum.org/content/37/10/2096.abstract
AB Objective. To analyze the frequency and phenotype of peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cell (SFMC) T helper (Th)17 cells in reactive arthritis (ReA). Methods. T cell surface phenotype and cytokine production were measured following stimulation, using 8-color flow cytometry. Results. The percentages of interleukin 17 (IL-17)–positive CD4+ T cells were increased in SFMC of patients with ReA compared with PBMC. All IL-17+ cells were CD4+CD45RO+; in SFMC most expressed CCR6, but only 50% expressed CCR4. IL-17+ cells sometimes coexpressed IL-22 and/or interferon-γ, but not IL-10. Conclusion. These data support the hypothesis that Th17 cells are involved in ReA pathogenesis.