PT - JOURNAL ARTICLE AU - HUI SHEN AU - JANE C. GOODALL AU - J.S. HILL GASTON TI - Frequency and Phenotype of T Helper 17 Cells in Peripheral Blood and Synovial Fluid of Patients with Reactive Arthritis AID - 10.3899/jrheum.100146 DP - 2010 Oct 01 TA - The Journal of Rheumatology PG - 2096--2099 VI - 37 IP - 10 4099 - http://www.jrheum.org/content/37/10/2096.short 4100 - http://www.jrheum.org/content/37/10/2096.full SO - J Rheumatol2010 Oct 01; 37 AB - Objective. To analyze the frequency and phenotype of peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cell (SFMC) T helper (Th)17 cells in reactive arthritis (ReA). Methods. T cell surface phenotype and cytokine production were measured following stimulation, using 8-color flow cytometry. Results. The percentages of interleukin 17 (IL-17)–positive CD4+ T cells were increased in SFMC of patients with ReA compared with PBMC. All IL-17+ cells were CD4+CD45RO+; in SFMC most expressed CCR6, but only 50% expressed CCR4. IL-17+ cells sometimes coexpressed IL-22 and/or interferon-γ, but not IL-10. Conclusion. These data support the hypothesis that Th17 cells are involved in ReA pathogenesis.