Article Figures & Data
Figures
- Figure 1.
Expression of omentin in synovial tissue of patients with RA or OA. Immunostaining of omentin in synovial tissue was done with nuclear counterstaining (hematoxylin). Nuclei are stained in blue, and omentin is stained in red. A primary antibody isotype control is shown for RA and OA synovial tissue in (E) and (F). Expression of omentin in synovial tissue was most prominent within the synovial lining layer (black arrowheads) and perivascular (white arrowheads). RA synovial tissue (A and B) showed the same expression pattern of omentin as OA synovial tissue (C and D). RA: rheumatoid arthritis; OA: osteoarthritis.
Tables
- Table 1.
Results of mRNA/protein expression analysis of omentin-stimulated RASF. RASF stimulated with 500 ng/ml omentin for 15 h and untreated controls were analyzed by Affymetrix (AFFX) oligonucleotide microarrays (GeneChip HG U133A) and protein arrays (RayBio “Custom Human Cytokine Antibody Array” and “Human Chemokine Antibody Array I”). The table shows a selection of functionally classified genes that according to the arrays were induced or repressed by omentin with fold changes of ≥ 2 (induction) or ≤ −2 (repression), respectively. For verification, selected genes/proteins were quantified by real-time PCR and ELISA.
Gene/Protein Symbol Fold Change AFFX Fold Change Real-time PCR Fold Change Protein Arrays Fold Change ELISA Chemokines Chemokine (C-C motif) ligand 3 (CCL3) MIP-1a −7.6 — 2.7 n.d. Chemokine (C-C motif) ligand 7 (CCL7) MCP-3 8.5 — 4.6 n.d. Chemokine (C-C motif) ligand 8 (CCL8) MCP-2 ≈ — 5.2 — Chemokine (C-C motif) ligand 18 (CCL18) PARC ≈ — 4.3 — Chemokine (C-C motif) ligand 20 (CCL20) CCK1 ≈ — 5.0 — Chemokine (C-X-C motif) ligand 9 (CXCL9) MIG ≈ — 5.5 n.d. Chemokine (C-X-C motif) ligand 10 (CXCL10) IP-10 −3.9 n.d. ≈ — Chemokine (C-X-C motif) ligand 11 (CXCL11) I-TAC −5.5 n.d. ≈ — Cytokines Interleukin 3 IL-3 4.5 — ≈ — Interleukin 11 IL-11 5.6 — Ø — Interleukin 17C IL-17C 3.7 — Ø — Tumor necrosis factor-α TNF-α ≈ — 4.7 n.d. Miscellaneous inflammatory molecules Prostaglandin endoperoxide synthase 1 PTGS1 4.8 — Ø — Pre-B cell growth and B cell activation Bone marrow stromal cell antigen 2 BST2 3.0 ≈ Ø — Receptors Chemokine (C-C) motif receptor 9 CCR9 −6.1 — Ø — Fibroblast growth factor receptor 3 FGFR3 3.7 — Ø — Proteinases and peptidases Matrix metallopeptidase 9 (gelatinase B) MMP9 3.2 — 3.0 — Matrix metallopeptidase 10 (stromelysin 2) MMP10 3.2 — 2.5 — Matrix metallopeptidase 14 MMP14 −4.0 — Ø — ECM and cell surface molecules Collagen, type XXIV, alpha 1 COL24A1 −13.9 — Ø — Collagen, type VII, alpha 1 COL7A1 −4.8 — Ø — Glypican-3 GPC3 13.1 — Ø — Bone and cartilage metabolism Bone morphogenetic protein 8b BMP8B 3.0 — Ø — Growth factors Endothelial cell growth factor 1 (platelet-derived) ECGF1 5.0 — Ø — Insulin-like growth factor 1 (somatomedin C) IGF-1 2.0 — 10.8 n.d. Vascular endothelial growth factor VEGF ≈ — 3.6 — RASF: rheumatoid arthritis synovial fibroblasts; ECM: extracellular matrix; ≈: no change (i.e., less than ± 2-fold change); Ø: not present on array; n.d.: not detectable.
