Article Figures & Data
Figures
- Figure 1.
Prevalence of anti-DFS70 autoantibodies as detected by CIA in samples grouped according to the ANA IIF patterns on HEp-2 cell substrates. DFS70: dense fine speckled 70 kDa antigen; CIA: chemiluminescence immunoassay; ANA: antinuclear antibody; IIF: indirect immunofluorescence.
Tables
Cohort n DFS70-positive, n DFS70-positive, % p† Reference cohorts, n = 345 ANA referrals, qCTD* 200 9 4.5 NS Healthy 145 3 2.1 NA AARD, n = 383 cSLE 331 19 5.7 NS JDM 11 2 18.2 NS Diffuse cSSc 22 1 4.5 NS Limited cSSc 19 0 0.0 NS Other inflammatory conditions, n = 360 JIA without uveitis 183 3 1.6 NS JIA with uveitis 19 2 10.5 NS Arthralgia 32 0 0.0 NS Celiac disease 49 2 4.1 NS Localized scleroderma 29 4 13.8 0.0310 Idiopathic uveitis 7 1 14.3 NS Reactive arthritis 21 0 0.0 NS Other** 20 0 0.0 NS ↵* Sera referred for an ANA/ENA test as part of the investigations for qCTD. Sera were from children ≤ 18 years of age.
↵** Cases of Crohn disease, Reiter syndrome, panniculitis, sarcoidosis, primary antiphospholipid syndrome, viral myopathy, eosinophilic fasciitis.
↵† P value is calculated against healthy individuals by Student t test. DFS70: dense fine speckled 70 kDa antigen; ANA: antinuclear antibody; AARD: ANA-associated rheumatic disease; qCTD: query connective tissue disease (children being investigated for an AARD); cSLE: childhood systemic lupus erythematosus; cSSc: childhood systemic sclerosis; JIA: juvenile idiopathic arthritis; NA: not applicable; NS: not statistically significant; JDM: juvenile dermatomyositis.
Patient No. Diagnosis Age, yrs Sex Racial Group DFS70 Reactivity by CIA, CU ANA Pattern/titer by IIF ENA/LIA/other Autoantibodies* 1 JIA 10 M White 36.3 NT Negative 2 JIA 13 M White 49.9 NT Negative 3 JIA 13 F White 27.9 DFS 1/320 Negative 4 JIA uveitis 6 F White 131.5 DFS 1/5120 Negative 5 JIA uveitis 9 M Asian 38.2 DFS 1/1280 Negative 6 Uveitis 16 M White 78.2 DFS 1/2560 Negative 7 cSLE 15 F White 212.0 DFS 1/1280 Sm, U1RNP weak positive 8 cSLE 16 F White 138.4 DFS 1/1280 dsDNA, U1RNP 9 cSLE 18 F Asian 88.6 DFS, MND dsDNA, U1RNP 10 cSLE 10 F Mixed 140.1 DFS 1/2560 Sm, U1RNP, Ro52 11 cSLE* 15 F White 59.1 DFS 1/2560 U1RNP, weak dsDNA 12 cSLE 14 F Black 45.6 DFS, NLR, cytoplasmic 1/1280 U1RNP, Ro60, weak Rib P, Ro52 13 cSLE 13 F Asian 39.1 DFS 1/320 Negative 14 cSLE* 15 F White 81.5 DFS, NLR 1/1280 U1RNP 15 cSLE 13 F Asian 30.7 DFS 1/2560 Sm, U1RNP, Ro60 16 cSLE 7 F Hispanic 71.1 DFS 1/2560 Sm, U1RNP, Ro60, Ro52, weak SS-B 17 cSLE 12 F White 194.0 DFS 1/2560 Negative 18 cSLE 14 M White 312.3 DFS 1/2560 Negative 19 cSLE 5 F Asian 140.7 DFS 1/1280 Negative 20 cSLE 18 F Asian 232.1 DFS 1/320 U1RNP 21 cSLE 17 F Unknown > 450.8 DFS 1/5120 Low dsDNA 22 cSLE 16 M Unknown 209.6 DFS 1/1280 Negative 23 cSLE 16 F White 22.1 Cytoplasmic 1/160 Negative 24 cSLE 16 F Asian 26.7 Homo, NLR 1/1280 Low U1RNP 25 cSLE 15 F Asian 35.6 DFS 1/640 Low U1RNP 26 LS 8 M White 120.5 DFS 1/640 Negative 27 LS 14 F White 60.1 MND, NLR, CS 1/1280 Negative 28 LS 15 M Mixed 35.9 DFS 1/320 Negative 29 LS 12 F Mixed 57.2 DFS, NLR 1/1280 Negative 30 cdSSc 18 F White 67.8 DFS, CENP 1/1280 CENP-B 31 JDM 6 F Asian 408.3 Negative Negative 32 CD 12 F White 51.4 DFS 1/1280 Anti-tTG–positive 33 CD 14 M White 67.1 DFS 1/1280 Anti-tTG–negative 34 qCTD 6 M White 115.8 DFS 1/2560 Negative 35 qCTD 7 F White 72.8 DFS 1/5120 Negative 36 qCTD 13 F White 286.3 DFS 1/5120 Anti-dsDNA 37 qCTD 7 F White 79.4 DFS 1/5120 Negative 38 qCTD 14 F White 326.9 DFS 1/5120 Negative 39 qCTD 5 M White 87.6 DFS 1/640 Negative 40 qCTD 10 M White 23.5 DFS 1/160 Negative 41 qCTD 7 F White 80.3 DFS 1/640 Negative 42 qCTD 9 F White 32.3 DFS 1/1280 NOR90 43 Healthy 9 M White 21.7 DFS NT 44 Healthy 7 F Asian 59.1 MND NT 45 Healthy 14 F White 26.1 DFS Negative ↵* Patients 11 and 14 are identical twins. DFS70: dense fine speckled 70 kDa antigen; CIA: chemiluminescence immunoassay; CU: chemiluminescence units; ANA: antinuclear antibody; IIF: indirect immunofluorescence; ENA: extractable nuclear antigens; LIA: line immunoassay; JIA: juvenile idiopathic arthritis; cSLE: childhood systemic lupus erythematosus; LS: localized scleroderma (morphea, linear scleroderma); cdSSc: childhood diffuse cutaneous systemic sclerosis; JDM: juvenile dermatomyositis; CD: celiac disease; qCTD: query connective tissue disease, children being investigated for an AARD; AARD: ANA-associated rheumatic disease; NT: not tested; MND: multiple nuclear dots; NLR: nucleolar; CS: centrosome; CENP: centromere protein; tTG: tissue transglutaminase.
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