Skip to main content

Main menu

  • Home
  • Content
    • First Release
    • Current
    • Archives
    • Collections
    • Audiovisual Rheum
    • COVID-19 and Rheumatology
  • Resources
    • Guide for Authors
    • Submit Manuscript
    • Payment
    • Reviewers
    • Advertisers
    • Classified Ads
    • Reprints and Translations
    • Permissions
    • Meetings
    • FAQ
    • Policies
  • Subscribers
    • Subscription Information
    • Purchase Subscription
    • Your Account
    • Terms and Conditions
  • About Us
    • About Us
    • Editorial Board
    • Letter from the Editor
    • Duncan A. Gordon Award
    • Privacy/GDPR Policy
    • Accessibility
  • Contact Us
  • JRheum Supplements
  • Services

User menu

  • My Cart
  • Log In

Search

  • Advanced search
The Journal of Rheumatology
  • JRheum Supplements
  • Services
  • My Cart
  • Log In
The Journal of Rheumatology

Advanced Search

  • Home
  • Content
    • First Release
    • Current
    • Archives
    • Collections
    • Audiovisual Rheum
    • COVID-19 and Rheumatology
  • Resources
    • Guide for Authors
    • Submit Manuscript
    • Payment
    • Reviewers
    • Advertisers
    • Classified Ads
    • Reprints and Translations
    • Permissions
    • Meetings
    • FAQ
    • Policies
  • Subscribers
    • Subscription Information
    • Purchase Subscription
    • Your Account
    • Terms and Conditions
  • About Us
    • About Us
    • Editorial Board
    • Letter from the Editor
    • Duncan A. Gordon Award
    • Privacy/GDPR Policy
    • Accessibility
  • Contact Us
  • Follow jrheum on Twitter
  • Visit jrheum on Facebook
  • Follow jrheum on LinkedIn
  • Follow jrheum on YouTube
  • Follow jrheum on Instagram
  • Follow jrheum on RSS
Research ArticleArticle

Association of Polymorphisms Modulating Low-density Lipoprotein Cholesterol with Susceptibility, Severity, and Progression of Rheumatoid Arthritis

Yune-Jung Park, Seung-Ah Yoo, Susanna Choi, Hee-Soo Yoo, Ho-Sung Yoon, Chul-Soo Cho, Ki-Dong Yoo and Wan-Uk Kim
The Journal of Rheumatology June 2013, 40 (6) 798-808; DOI: https://doi.org/10.3899/jrheum.120954
Yune-Jung Park
From the Department of Internal Medicine, The Catholic University of Korea, School of Medicine, and the Research Institute of Immunobiology, The Catholic University of Korea, Gyeonggi-do, Korea.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Seung-Ah Yoo
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Susanna Choi
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hee-Soo Yoo
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ho-Sung Yoon
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chul-Soo Cho
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ki-Dong Yoo
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: wan725@catholic.ac.kr yookd@catholic.ac.kr
Wan-Uk Kim
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: wan725@catholic.ac.kr yookd@catholic.ac.kr
  • Article
  • Figures & Data
  • References
  • Info & Metrics
  • PDF
  • eLetters
PreviousNext
Loading

Abstract

Objective. Dyslipidemia, a risk factor for cardiovascular diseases, is more prevalent in patients with rheumatoid arthritis (RA) than in the general population. We investigated whether single-nucleotide polymorphisms (SNP) modulating low-density lipoprotein (LDL) cholesterol affect susceptibility, severity, and progression of RA.

Methods. We enrolled 302 patients with RA and 1636 healthy controls, and investigated the SNP modulating LDL cholesterol. Clinical characteristics of RA, serum adipocytokine concentrations, and radiographic severity were analyzed according to genotype score based on the number of unfavorable alleles. The influence of genotype score on radiographic progression was also investigated using multivariable logistic models.

Results. We identified 3 SNP (rs688, rs693, and rs4420638) modulating LDL cholesterol in Koreans, which correlated well with LDL cholesterol levels in both patients with RA and controls. Among them, 2 SNP, rs688 and rs4420638, were more prevalent in patients with RA than in controls. In patients with RA carrying more unfavorable alleles (genotype score ≥ 3), disease activity measures, serum adipocytokine levels, and radiographic severity were all increased. The genotype score was an independent risk factor for radiographic progression of RA over 2 years, and its effect was greater than the influence of conventional risk factors.

Conclusion. SNP modulating LDL cholesterol influence the risk, activity, and severity of RA. These results provide the first evidence that genetic mechanisms linked to dyslipidemia may directly contribute to the susceptibility and prognosis of RA, a representative of chronic inflammatory diseases, explaining the high incidence of dyslipidemia in RA.

Key Indexing Terms:
  • RHEUMATOID ARTHRITIS
  • DYSLIPIDEMIA
  • POLYMORPHISMS
  • RADIOGRAPHIC PROGRESSION

The main cause of death for patients with rheumatoid arthritis (RA) is cardiovascular disease (CVD)1. Dyslipidemia, one of the most important risk factors for CVD, is more prevalent in patients with RA than in the general population2. Since RA is a chronic inflammatory disease, it has been suggested that the high inflammatory state associated with RA may induce dyslipidemia2. For example, high disease activity is related to unfavorable lipid profile3, and control of inflammation by antirheumatic drugs conversely improves dyslipidemia in patients with RA4. However, evidence is emerging that dyslipidemia is already present years before the advent of arthritis5. This suggests that dyslipidemia in patients with RA cannot be explained simply by hyperinflammation per se, and that other factors also contribute to development of dyslipidemia in RA.

It is estimated that up to 50% of plasma levels of low-density lipoprotein (LDL) cholesterol is heritable in the general population6. Because RA is a disease with a strong genetic etiology7,8, increased LDL concentration in patients with RA, which is critical for accelerated atherosclerosis, might be dependent on a genetic mechanism(s). Indeed, it is reported that the RA susceptibility gene is related to dyslipidemia9. The lymphotoxin-α gene, a proinflammatory cytokine gene, is an independent risk factor for dyslipidemia as well as for susceptibility to RA10. It has been also demonstrated that apolipoprotein E genotypes are strongly associated with inflammation and lipid levels in RA11. Recently, we reported the association of the APOM C-1065A polymorphism with an increased risk for developing RA and dyslipidemia in patients with RA12, and suggested that genetic risk factors for dyslipidemia might play a causal role in RA susceptibility. However, no evidence has been found that such polymorphisms directly affect the risk and phenotype of RA.

Interestingly, lipids have a direct modulating effect on inflammation. Several animal studies have reported that hypercholesterolemia induces inflammation by increasing circulating inflammatory cells13,14. Additionally, it is suggested that dyslipidemia, which is noted frequently in patients with the metabolic syndrome or obesity, should be considered a chronic inflammatory state15. Similar evidence has also been found in arthritic conditions. For example, patients with osteoarthritis with large infrapatellar fat pad show an inflammatory phenotype16. It has been documented that RA patients who have a higher level of circulating adipocytokine, a proinflammatory fat-derived hormone, exhibit more severe joint destruction than patients with lower serum adipocytokines17,18. Therefore, it is possible that dyslipidemia itself, such as hyper-LDL cholesterolemia, may be causally associated with inflammatory activity of RA, culminating in joint destruction.

We formulated the following hypotheses. First, there may be common genetic mechanisms that can simultaneously increase the susceptibility of both dyslipidemia and RA. Second, because cholesterol itself can directly influence inflammation14,15, LDL cholesterol-associated polymorphisms might affect the clinical features of RA, including disease activity. Third, given that a certain gene may influence a disease phenotype throughout life, these polymorphisms could affect the severity of RA as a result of cumulative inflammatory injury. To address these issues, we investigated LDL cholesterol-associated polymorphisms in patients with RA and their association with susceptibility, activity, and radiographic severity of RA.

MATERIALS AND METHODS

Patients and study samples

The Vincent Arthritis Study is a hospital-based prospective cohort of consecutive patients with RA. All participants fulfilled the 1987 American College of Rheumatology criteria for the classification of RA19. From this cohort, 302 patients with RA were recruited; all provided written informed consent and received baseline examinations between 2007 and 2010. The non-RA healthy controls consisted of subjects from the Ansung and Ansan cohort. This cohort, representing rural (Ansung) and urban (Ansan) communities, was established in 2001 as a part of the Korean Genome Epidemiology Study (KoGES)20. From this cohort, 1636 age- and sex-matched subjects were selected randomly.

All subjects underwent a clinical and laboratory evaluation. Information was collected for the following: age, sex, body mass index (BMI), smoking status, hypertension, and diabetes. Cigarette smoking status was elicited by a self-administered questionnaire and current smoking was defined as any smoking within the past year. Hypertension was defined as average systolic blood pressure ≥ 140 mm Hg and diastolic blood pressure ≥ 90 mm Hg or use of antihypertensive medications. Diabetes was defined as a fasting glucose level ≥ 126 mg/dl, nonfasting glucose level ≥ 200 mg/dl, or the use of glucose-lowering medications.

In patients with RA, the following information was also collected: disease duration, positivity for rheumatoid factor (RF), positivity for anticitrullinated protein antibody (ACPA), disease activity, and disease severity. Disease activity was evaluated with a Disease Activity Score 28-joint assessment (DAS28)21. Disease severity was assessed by evaluating radiographic damage on hand and foot radiographs. The study protocol was approved by the Institutional Review Board of the Catholic Medical Center (XC09TIMI0070).

Genotyping

Genomic DNA from patients was prepared from whole blood samples using the DNeasy Blood & Tissue Kit (Qiagen Sample & Assay Technologies). The genotypes of 3 single nucleotide polymorphisms (SNP; rs688, rs693, and rs4420638) modulating LDL cholesterol were ascertained by PCR amplification of genomic DNA using sequence-specific forward and reverse primers, determined using a BigDye® Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems) according to the manufacturer’s protocol, and analyzed on an ABI-3730 apparatus (Applied Biosystems). Genotyping data for control non-RA subjects were obtained from the Korean Association Resource KARE project, which included genotypes of 8842 of 10,038 participants using Affymetrix Genome-Wide Human SNP Arrays 5.0.

Selection of SNP

We wanted to identify SNP modulating LDL cholesterol in Korean patients with RA on the basis of the literature as well as from a genome-wide association study performed in a Korean population. We first selected 7 SNP (in 5 genes) established in previous studies22,23,24,25,26, all of which showed a close association with plasma LDL cholesterol levels in subjects of European ancestry. These include APOB (apolipoprotein B, rs693 and rs7575840), APOE cluster (apolipoprotein E, rs4420638), HMGCR (3-hydroxy-3-methylglutaryl-coenzyme A reductase, rs12654264), LDLR (low-density lipoprotein receptor, rs688 and rs1529729), and PCSK9 (proprotein convertase subtilisin/kexin type 9, rs11591147). To select SNP modulating LDL cholesterol applicable to the Korean population, we next analyzed a genotype database constructed as part of the KARE project20, which included genotypes of 10,038 participants using Affymetrix Genome-Wide Human SNP Arrays 5.0. As a result, only 3 (rs693, rs688, and rs4420638) out of the 7 SNP were identified as having a good correlation with plasma concentrations of LDL cholesterol in Koreans27, and were subjected to further analysis for our study.

Radiographic assessment

Radiographs of the hands and feet were taken at baseline and biannually thereafter. Radiographic severity was scored in chronologic order for erosions and joint space narrowing according to the Sharp-van der Heijde (SvdH) method28, and was determined by 2 board-certified physicians who were blinded to each patient’s identity and clinical status. The interobserver variability described by the interclass correlation coefficient was 0.91. Joint space narrowing and erosion scores were summed to give the total radiographic progression score. Erosion and narrowing progression scores were calculated by subtracting the initial score from the score after a 2-year followup. Radiographic progression was defined as progression score ≥ 417.

Measurement of plasma lipid levels and adipocytokine levels

Lipid variables were measured in fasting venous blood samples according to standard procedures at the Department of Clinical Chemistry, St. Vincent’s Hospital, The Catholic University of Korea. Plasma total cholesterol (TC) and triglyceride were assayed by enzymatic methods. High-density lipoprotein (HDL) cholesterol was measured with a homogenous enzymatic colorimetric test. LDL cholesterol was calculated by the Friedewald formula, with assignment of missing values to subjects with a triglyceride level < 400 mg/dl. We considered patients to have dyslipidemia if their fasting plasma HDL cholesterol was < 40 mg/dl in men and < 50 mg/dl in women, or LDL cholesterol was ≥ 160 mg/dl, or triglyceride was ≥ 200 mg/dl, or TC ≥ 200 mg/dl. Serum samples were obtained at baseline and stored at −70°C. The concentrations of adipocytokines in supernatants [leptin (ng/ml), adiponectin (μg/ml), and interleukin 6 (IL-6; pg/ml)] were measured using a commercial ELISA kit (R&D Systems) according to the manufacturer’s recommendations.

Statistical analyses

The distributions of all variables were examined. Comparisons were performed by independent t test or chi-square tests for means and proportions between patients and controls. Consistent with a previous study25, for each subject levels of LDL cholesterol were adjusted for age, sex, and the presence of diabetes to create a multivariable-adjusted residual LDL cholesterol level. Control subjects who were treated with lipid-lowering medication were excluded. We used a logistic regression model to estimate OR for RA susceptibility after adjusting for age, sex, presence of autoantibody, and smoking status. To evaluate the ability of the genotype score to predict risk of radiographic progression, we plotted receiver-operating characteristic (ROC) curves for the genotype score with covariates. We used radiographic progression events (any increase in SvdH score ≥ 4) as the outcome after determining the presence of radiographic progression (any increase in SvdH score ≥ 4) at 2 years. All p values are 2-tailed, with a p value of 0.05 indicating statistical significance. Based on 90% power (1 – β) at a 2-sided error α = 0.05 to detect the difference (rs4420638, 5.3 mg/dl; rs688, 6.3 mg/dl; and rs693, 15.3 mg/dl), 275 patients with RA and 1382 control subjects were needed. Allowing for 10% attrition over the study period, at least 303 RA and 1520 healthy subjects needed to be recruited. Analyses were performed with the use of SPSS software, version 13.0 (SPSS) or R software.

RESULTS

Baseline characteristics

Demographic and clinical characteristics in the study participants are summarized in Table 1. We compared demographic data of patients with RA (n = 302) and healthy controls (n = 1636). The mean age of patients with RA was 53.5 ± 12.3 years and 78.5% were women. Patients with RA had more dyslipidemia but lower BMI than controls (p < 0.001, respectively; Table 1). One hundred sixty-eight patients (55.6%) received methotrexate, 177 (58.6%) hydroxychloroquine, 28 (9.3%) anti-tumor necrosis factor-α (TNF-α) antibodies, and 204 (67.5%) patients were treated with a low dose of prednisolone (≤ 10 mg).

View this table:
  • View inline
  • View popup
Table 1.

Demographic features of patients with rheumatoid arthritis (RA) and controls. Data are presented as mean ± SD, median (interquartile range), or percentage as appropriate.

Validation of LDL cholesterol levels

Data on the distribution of genotypes and alleles in participants are presented in Table 2. The genotype frequency of each LDL cholesterol polymorphism agreed with that predicted by the Hardy-Weinberg equation in both patients with RA and controls (p > 0.05). We compared the LDL cholesterol levels according to each SNP. In controls carrying the unfavorable allele that was associated with high LDL cholesterol levels, the difference in LDL cholesterol level between homozygote classes ranged from 5.8 to 17.4 mg/dl. In patients with RA, LDL cholesterol levels were increased in those carrying the minor allele, ranging from 14.1 to 31.4 mg/dl. However, there were too few subjects carrying the minor allele in rs693, and there was no significant trend toward LDL cholesterol levels between homozygote classes. The influence of the 2 SNP on LDL cholesterol levels remained significant after correction for age, sex, and medications (data not shown).

View this table:
  • View inline
  • View popup
Table 2.

Validation of low-density lipoprotein (LDL) cholesterol level according to single-nucleotide polymorphism (SNP)-associated LDL cholesterol in patients with RA and controls.

SNP and RA susceptibility

The frequency of unfavorable (minor) alleles linked to hyper-LDL cholesterolemia was significantly higher in patients with RA than in controls (p = 0.001 for rs688, p = 0.003 for rs4420638). In particular, findings for rs4420638 remained significant after the stratified analysis according to the presence of dyslipidemia (p < 0.001 for the dyslipidemic group, p = 0.001 for the nondyslipidemic group, data not shown). We further compared patients with RA to control subjects with diabetes mellitus, which is known to be more prevalent in those with dyslipidemia, to exclude the carryover effect. One hundred forty-one (51.6%) diabetic patients had dyslipidemia. Interestingly, there was no difference in LDL cholesterol levels between patients with RA and those with diabetes mellitus (131.2 ± 36.3 mg/dl in RA vs 134.8 ± 26.9 mg/dl in diabetes mellitus; p = 0.062), but the frequency of the minor allele was significantly higher in RA than in diabetes mellitus (rs688, p = 0.044; rs4420638, p = 0.002; Appendix 1). The influence of the 2 SNP on the risk of developing RA was still statistically significant after correction for age, sex, and the presence of dyslipidemia [OR 1.988 (95% CI 1.158–2.346) for rs688 and OR 1.327 (95% CI 1.241–3.648) for rs4420638, data not shown]. However, in a fully adjusted model, this significance was lost. The minor allele homozygote of rs4420638 increased the susceptibility for RA, regardless of the autoantibody status, and the rs688 minor homozygotes increased the risk of developing RA, limited to seropositive patients (Figure 1A, 1B). Similarly, among current smokers, patients with the minor allele homozygote of rs688, but not that of rs4420638, exhibited a significant increase in the risk of RA (Figure 1C).

Figure 1.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 1.

Low-density lipoprotein (LDL) cholesterol-associated polymorphisms and susceptibility to rheumatoid arthritis (RA). A–C. OR for developing RA. OR are calculated per single unfavorable allele, compared with major allele homozygotes. A and B. OR according to the presence or absence of anticitrullinated protein antibody (ACPA) or rheumatoid factor (RF). C. OR according to smoking status. D. RA susceptibility according to the genotype score. Symbols and lines represent OR and 95% CI, respectively. The logistic-regression model is adjusted for age, sex, and the presence of dyslipidemia. OR for RA susceptibility are calculated per single unfavorable allele as compared with genotype score = 0. *p < 0.05.

Genotype score and RA susceptibility

In line with a previous study25, we constructed a genotype score for each patient by counting the number of minor alleles. This scoring system is based on the assumption that the combination of involved polymorphisms would be more relevant to explain the phenotype of a certain disease than a single SNP, which reflects only a modest fraction of the variance in phenotype29. With zero, 1, or 2 minor alleles for each SNP, the possible genotype score ranged from 0 to 6. Indeed, the absolute differences in LDL cholesterol levels (57.9 mg/dl) were greater than those of each SNP (14.1 to 31.4 mg/dl). Because a small number (n = 24) of patients with RA had a score of 3 or more, these patients were regarded as a single group. As shown in Appendix 2, with a higher genotype score, plasma levels of LDL cholesterol were proportionally increased in both RA patients and controls, even after adjustment for age and sex. Moreover, subjects with a genotype score ≥ 3 had a higher risk for RA, by 4.12-fold, compared to healthy controls (Figure 1D).

Genotype score, RA activity, and RA severity

Next, we investigated whether each genotype influences RA activity and severity. RA patients carrying minor allele homozygotes of rs688 had higher levels of disease activity and those carrying rs4420638 showed a trend toward higher radiographic score (Appendix 3). However, the significance of the relationship between each SNP and RA disease activity/severity disappeared after adjustment for disease duration, presence of autoantibody, and therapeutic options (data not shown). Interestingly, when genotype score, rather than a single SNP, was introduced for the analysis, its association with disease activity and radiographic severity became statistically significant (Table 3). For example, the erosion score, narrowing score, and total SvdH score all increased proportionally as genotype score increased (Table 3). To ascertain the relationship between genotype score and radiographic severity, we performed additional exploratory analyses, considering other traditional risk factors that affect radiographic severity of RA (Appendix 4), and as described in other studies30. After adjustment for these confounders and medications, genotype score was still independently associated with radiographic severity (β coefficient = 0.162, p = 0.004; Figure 2A). Because genotype score correlated well with the LDL cholesterol level, we next investigated whether plasma LDL cholesterol concentrations reflect radiographic severity.

Figure 2.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 2.

Association of polymorphisms modulating LDL cholesterol levels with radiographic severity and progression in RA. A. Multivariable analysis of the association between genotype score and radiographic severity. Adjusted R2 for the model is 24.9%. This model is adjusted for age, body mass index, sex, ESR, CRP, RF positivity, use of medications (glucocorticoid, methotrexate, hydroxychloroquine, anti-TNF-α, and statin), and dyslipidemia. B. Radiographic severity 2 years later according to plasma LDL cholesterol levels. Patients with high LDL cholesterol group (3rd tertile) had higher radiographic score than 1st tertile group. *p < 0.05. C. Receiver-operating characteristic (ROC) curves for radiographic progression during 2-year followup. D. Genotype score as a predictor for radiographic progression over 2 years. For adjusted OR, logistic regression was used after adjustment for age, smoking status, RF positivity, ACPA positivity, platelet levels, ESR levels, CRP levels, use of medications (glucocorticoid, methotrexate, hydroxychloroquine, anti-TNF-α, and statin), body mass index, and dyslipidemia. LDL: low-density lipoprotein; RA: rheumatoid arthritis; ESR: erythrocyte sedimentation rate; CRP: C-reactive protein; RF: rheumatoid factor; TNF: tumor necrosis factor; ACPA: anticitrullinated protein antibody.

View this table:
  • View inline
  • View popup
Table 3.

Comparison of disease activity, radiographic severity, autoantibody, and medications according to genotype score. The genotype score represents the number of unfavorable alleles (the allele associated with higher LDL cholesterol) at each of 3 single-nucleotide polymorphisms.

Genotype score and radiographic progression

Based on our finding that genotype score was involved in radiographic severity, we further investigated whether this score could serve as a predictor of radiographic progression. When the prevalence of cholesterol-raising SNP in RA patients with and those without radiographic progression was compared, patients carrying the minor allele of rs688 and rs4420638 were observed to have radiographic progression (Appendix 5). In agreement with previous reports31,32, we observed that at baseline, platelet count, erythrocyte sedimentation rate (ESR), RF titer, and smoking status were significant indicators for predicting radiographic progression in our cohort (Appendix 6), which was defined as progression score ≥ 4. Using these variables, we constructed ROC curves to determine the relative contribution of genotype score to radiographic progression in comparison to other conventional risk factors. Surprisingly, the area under the ROC curve derived from genotype score was 0.704 (Figure 2C), which was greater than that from other risk factors, including ESR/C-reactive protein (CRP) at baseline, the presence of RF or ACPA, and current smoking (Figure 2C). Moreover, the effect of genotype score on radiographic progression remained significant when it was subjected to additional adjustment for age, presence of dyslipidemia, and treatment option [OR 2.175 (95% CI 1.448–3.049); Figure 2D]. Together, these observations suggest that genotype score might be one of the important indicators to predict radiographic progression of RA.

Genotype score and adipocytokine levels

Finally, we wondered how genotype score was related to severity and progression of RA. Evidence is emerging that adipocytokines with proinflammatory activity, produced mainly from adipose tissues, are increased in patients with RA, and their levels correlate with disease activity and radiographic severity17,18. Thus, we measured circulating adipocytokine levels in sera samples of 246 patients with RA and compared them with genotype score and radiographic severity. The results showed that patients with higher genotype score (≥ 3) had elevated adipocytokine levels compared to patients with genotype score = 0 after adjustment for age, sex, and BMI (adiponectin, p < 0.001; leptin, p < 0.001; and IL-6, p = 0.041; Figure 3). Moreover, adiponectin and IL-6 levels correlated positively with radiographic scores (adiponectin, γ = 0.151, p = 0.033; IL-6, γ = 0.148, p = 0.036). However, leptin levels showed a trend for a positive correlation with the joint space narrowing score, although this was not statistically significant (γ = 0.141, p = 0.053). Collectively, these data suggest that the adipocytokine level varies depending on the genotype score, which might explain the destructive phenotype in RA patients with minor alleles.

Figure 3.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 3.

Correlation of genotype score with adipocytokine levels. Levels of adiponectin (A), interleukin (IL) 6 (B), and leptin (C) in the sera of patients with rheumatoid arthritis (n = 246) were determined by ELISA. Data show median ± SEM. *p < 0.05 versus genotype score 0.

DISCUSSION

Sometimes, 2 ostensibly unconnected conditions are closely related. Examples include Alzheimer’s disease and dyslipidemia33, baldness and coronary heart disease34, and height and diabetes or cancer35. This interrelationship may be a genetic factor. The ε4 allele of the apolipoprotein E gene (APOE-ε4) has been confirmed as a genetic risk factor for late-onset Alzheimer’s disease and dyslipidemia36. The high mobility group A gene associated with height frequently affects cancer, obesity, atherosclerosis, and diabetes35. In our study, we discovered that polymorphisms associated with LDL cholesterol (rs688 and rs4420638) previously thought to be unrelated are actually involved in susceptibility to RA. Moreover, they can be a good predictor for RA disease activity, severity, and progression as well as plasma cholesterol levels. To our knowledge, this is the first study to show the association of cholesterol-related polymorphisms with RA susceptibility, severity, and prognosis.

In our study, we created a genotype score from 3 validated SNP modulating LDL cholesterol, consistent with prospective studies of European ancestry23. Constructing a genotype score is known to be more effective in explaining some diseases or phenomena because each polymorphism acts independently and additively on the risk of disease, whereas a single-sequence variant is limited to explaining only a modest fraction of the variance (2% or less)29. Similar to studies conducted in diabetes, schizophrenia, bipolar disorder, and CVD25,37,38, we found that the genotype score in RA displayed a stronger clinical relevance than a single SNP. In particular, the association of the genes with disease activity and severity became significant in analysis of the genotype score, although it was not evident with individual SNP, indicating that a combination of the involved SNP should be investigated to identify genetic mechanisms of the development and progression of RA.

Increasing evidence9,10 indicates that genetic factors are related to the presence of dyslipidemia in RA. Yet these studies are limited in that they explained a high prevalence of dyslipidemia primarily in association with proinflammatory cytokine genes. Thus, it remains to be determined why patients with preclinical RA have dyslipidemia7, and whether dyslipidemia plays a causal role in RA. We observed that the risk of RA and plasma LDL cholesterol levels increased proportionally as genotype score increased. This indicates that there should be a common genetic predisposition for the synchronicity of RA and dyslipidemia.

We also identified that genotype score was an independent risk factor for joint destruction. Importantly, it correlated well with radiographic progression over a period of up to 2 years, independent from the presence of ACPA, disease duration, and disease activity. How does the LDL cholesterol genotype score contribute to the radiographic severity of RA? Previous studies demonstrated that obese people are at increased risk of developing RA39. Also, 2 recent studies40,41 reported that lipid-lowering therapy may be protective against development of RA, reducing disease activity and the number of swollen joints. An animal study demonstrated that hypercholesterolemia interferes with the bone marrow stromal cell-derived factor-1/CXCR-4 axis, resulting in lymphocytosis, thrombocytosis, and progenitor cell mobilization42. Further, hypercholesterolemia increases circulating inflammatory monocyte counts and renders these cells more prone to emigration into target lesions13, indicating that dyslipidemia itself contributes to the inflammatory response. In our study, as the genotype score increased, measures of RA disease activity, including ESR/CRP, serum IL-6 level, and DAS28 score, all increased. This is in agreement with studies43 showing that hyperlipidemic patients have more inflammation than nondyslipidemic patients. Moreover, mean LDL cholesterol levels, which we found increased with genotype score (Table 2), correlated positively with radiographic severity, suggesting that polymorphisms modulating LDL cholesterol might contribute to rheumatoid inflammation, possibly by regulating plasma cholesterol levels.

Another possible explanation is that polymorphisms modulating LDL cholesterol may affect adipocytokine levels. Recently, adipocytokines have gained attention related to the pathogenesis of RA. It has been proven experimentally that leptin and adiponectin, as representative adipocytokines, were able to induce production of inflammatory mediators such as TNF-α and IL-6, promote T cell activation, and upregulate matrix metalloproteinase44,45. Concentrations of adipocytokines are increased in RA and correlate with disease activity, erosion, and joint space narrowing46,47. Our patients with a higher genotype score had elevated adipocytokine levels compared to those with a lower genotype score after adjustment for age, sex, and BMI. We also found that adiponectin and IL-6 levels were higher in patients with a higher radiographic score (data not shown). These findings, together with previous reports45,46,47, imply that increased adipocytokines, according to genotype score, could contribute to joint destruction.

Our study has some limitations. First, we analyzed only LDL cholesterol levels. Because other lipid profiles (e.g., HDL cholesterol) may also be involved in inflammatory activity, they should be considered a part of the genotype score. Second, we analyzed relatively few SNP, because only 3 SNP were finally confirmed in the process of selecting polymorphisms modulating LDL cholesterol established well in Europeans and of filtering SNP for Korean population through GWAS. Further, among them, rs693 had a much lower frequency in Korean subjects (minor allele frequency was 0.053 in the general population and 0.048 in patients with RA) compared to Europeans (frequency 0.40–0.48). Similarly to the finding that HLA-DR3, known to be an RA susceptibility allele in those of European descent, was rare in an Asian RA cohort48, SNP modulating LDL cholesterol levels seemed to show ethnic differences. Third, the 2-year followup period was too short to reveal development of new cardiovascular events and so we could not include this issue as a subsidiary theme. Clarification of this important issue is required.

Our study provides 3 important assertions. First, patients with RA have a dyslipidemic genetic predisposition. Second, genetic predisposition to LDL cholesterol levels, estimated by a genotype score, is associated with an increased risk of RA, which may explain why RA patients are more prone to CVD. Finally, acting together, SNP modulating LDL cholesterol levels may have a substantial influence on the inflammatory process, promoting radiographic severity of RA. Our findings suggest that, in addition to conventional risk factors, the LDL cholesterol-associated genotype score may be a notable marker for prediction of radiographic progression in patients with RA.

Appendix 1

View this table:
  • View inline
  • View popup

Comparison of single-nucleotide polymorphism (SNP)-associated low-density lipoprotein (LDL) cholesterol levels in patients with rheumatoid arthritis (RA) and diabetes mellitus.

Appendix 2

Figure
  • Download figure
  • Open in new tab
  • Download powerpoint

Low-density lipoprotein (LDL) cholesterol genotype score and plasma LDL cholesterol levels in patients with rheumatoid arthritis (RA) and in healthy subjects. Solid lines indicate spline plot of LDL cholesterol levels and broken lines are interquartile ranges. Data were adjusted for age, sex, and presence of diabetes.

Appendix 3

View this table:
  • View inline
  • View popup

Comparison of disease activity, radiographic severity, and disease phenotype according to each single-nucleotide polymorphism (SNP).

Appendix 4

View this table:
  • View inline
  • View popup

Association between radiographic severity score and risk factors.

Appendix 5

View this table:
  • View inline
  • View popup

Comparison of frequency of single-nucleotide polymorphisms (SNP) and genotype score between radiographic progression group and nonprogression group.

Appendix 6

View this table:
  • View inline
  • View popup

Association between radiographic progression score and risk factors.

Footnotes

  • Supported by grants from the Korea Healthcare Technology R&D Project, the Ministry for Health, Welfare and Family Affairs (No. A092258), the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (R33-2008-000-10064-0 and 2009-0080087), and the St. Vincent Hospital, Research Institute of Medical Science (SVHR-2012-04).

  • Accepted for publication December 21, 2012.

REFERENCES

  1. 1.↵
    1. Wolfe F,
    2. Mitchell DM,
    3. Sibley JT,
    4. Fries JF,
    5. Bloch DA,
    6. Williams CA,
    7. et al.
    The mortality of rheumatoid arthritis. Arthritis Rheum 1994;37:481–94.
    OpenUrlCrossRefPubMed
  2. 2.↵
    1. Choy E,
    2. Sattar N
    . Interpreting lipid levels in the context of high-grade inflammatory states with a focus on rheumatoid arthritis: A challenge to conventional cardiovascular risk actions. Ann Rheum Dis 2009;68:460–9.
    OpenUrlAbstract/FREE Full Text
  3. 3.↵
    1. Park YB,
    2. Lee SK,
    3. Lee WK,
    4. Suh CH,
    5. Lee CW,
    6. Lee CH,
    7. et al.
    Lipid profiles in untreated patients with rheumatoid arthritis. J Rheumatol 1999;26:1701–4.
    OpenUrlPubMed
  4. 4.↵
    1. Peters MJ,
    2. Vis M,
    3. van Halm VP,
    4. Wolbink GJ,
    5. Voskuyl AE,
    6. Lems WF,
    7. et al.
    Changes in lipid profile during infliximab and corticosteroid treatment in rheumatoid arthritis. Ann Rheum Dis 2007;66:958–61.
    OpenUrlAbstract/FREE Full Text
  5. 5.↵
    1. van Halm VP,
    2. Nielen MM,
    3. Nurmohamed MT,
    4. van Schaardenburg D,
    5. Reesink HW,
    6. Voskuyl AE,
    7. et al.
    Lipids and inflammation: Serial measurements of the lipid profile of blood donors who later developed rheumatoid arthritis. Ann Rheum Dis 2007;66:184–8.
    OpenUrlAbstract/FREE Full Text
  6. 6.↵
    1. Namboodiri KK,
    2. Kaplan EB,
    3. Heuch I,
    4. Elston RC,
    5. Green PP,
    6. Rao DC,
    7. et al.
    The Collaborative Lipid Research Clinics Family Study: Biological and cultural determinants of familial resemblance for plasma lipids and lipoproteins. Genet Epidemiol 1985;2:227–54.
    OpenUrlCrossRefPubMed
  7. 7.↵
    1. Terao C,
    2. Ohmura K,
    3. Kochi Y,
    4. Ikari K,
    5. Maruya E,
    6. Katayama M,
    7. et al.
    A large-scale association study identified multiple HLA-DRB1 alleles associated with ACPA-negative rheumatoid arthritis in Japanese subjects. Ann Rheum Dis 2011;70:2134–9.
    OpenUrlAbstract/FREE Full Text
  8. 8.↵
    1. de Vries RR,
    2. van der Woude D,
    3. Houwing JJ,
    4. Toes RE
    . Genetics of ACPA-positive rheumatoid arthritis: The beginning of the end? Ann Rheum Dis 2011;70 Suppl 1:i51–4.
    OpenUrlAbstract/FREE Full Text
  9. 9.↵
    1. Toms TE,
    2. Panoulas VF,
    3. Smith JP,
    4. Douglas KM,
    5. Metsios GS,
    6. Stavropoulos-Kalinoglou A,
    7. et al.
    Rheumatoid arthritis susceptibility genes associate with lipid levels in patients with rheumatoid arthritis. Ann Rheum Dis 2011;70:1025–32.
    OpenUrlAbstract/FREE Full Text
  10. 10.↵
    1. Santos MJ,
    2. Fernandes D,
    3. Caetano-Lopes J,
    4. Perpetuo IP,
    5. Vidal B,
    6. Canhao H,
    7. et al.
    Lymphotoxin-alpha 252 A>G polymorphism: A link between disease susceptibility and dyslipidemia in rheumatoid arthritis? J Rheumatol 2011;38:1244–9.
    OpenUrlAbstract/FREE Full Text
  11. 11.↵
    1. Toms TE,
    2. Smith JP,
    3. Panoulas VF,
    4. Blackmore H,
    5. Douglas KM,
    6. Kitas GD
    . Apolipoprotein E gene polymorphisms are strong predictors of inflammation and dyslipidemia in rheumatoid arthritis. J Rheumatol 2012;39:218–25.
    OpenUrlAbstract/FREE Full Text
  12. 12.↵
    1. Park YJ,
    2. Yoo SA,
    3. Lee JH,
    4. Chung YJ,
    5. Cho CS,
    6. Kim WU
    . The APOM polymorphism is a novel risk factor for dyslipidaemia in rheumatoid arthritis: A possible shared link between disease susceptibility and dyslipidaemia. Clin Exp Rheumatol 2012 Nov 28. [E-pub ahead of print]
  13. 13.↵
    1. Swirski FK,
    2. Libby P,
    3. Aikawa E,
    4. Alcaide P,
    5. Luscinskas FW,
    6. Weissleder R,
    7. et al.
    Ly-6Chi monocytes dominate hypercholesterolemia-associated monocytosis and give rise to macrophages in atheromata. J Clin Invest 2007;117:195–205.
    OpenUrlCrossRefPubMed
  14. 14.↵
    1. Drechsler M,
    2. Megens RT,
    3. van Zandvoort M,
    4. Weber C,
    5. Soehnlein O
    . Hyperlipidemia-triggered neutrophilia promotes early atherosclerosis. Circulation 2010;122:1837–45.
    OpenUrlAbstract/FREE Full Text
  15. 15.↵
    1. Gregor MF,
    2. Hotamisligil GS
    . Inflammatory mechanisms in obesity. Annu Rev Immunol 2011;29:415–45.
    OpenUrlCrossRefPubMed
  16. 16.↵
    1. Klein-Wieringa IR,
    2. Kloppenburg M,
    3. Bastiaansen-Jenniskens YM,
    4. Yusuf E,
    5. Kwekkeboom JC,
    6. El-Bannoudi H,
    7. et al.
    The infrapatellar fat pad of patients with osteoarthritis has an inflammatory phenotype. Ann Rheum Dis 2011;70:851–7.
    OpenUrlAbstract/FREE Full Text
  17. 17.↵
    1. Giles JT,
    2. van der Heijde DM,
    3. Bathon JM
    . Association of circulating adiponectin levels with progression of radiographic joint destruction in rheumatoid arthritis. Ann Rheum Dis 2011;70:1562–8.
    OpenUrlAbstract/FREE Full Text
  18. 18.↵
    1. Klein-Wieringa IR,
    2. van der Linden MP,
    3. Knevel R,
    4. Kwekkeboom JC,
    5. van Beelen E,
    6. Huizinga TW,
    7. et al.
    Baseline serum adipokine levels predict radiographic progression in early rheumatoid arthritis. Arthritis Rheum 2011;63:2567–74.
    OpenUrlCrossRefPubMed
  19. 19.↵
    1. Arnett FC,
    2. Edworthy SM,
    3. Bloch DA,
    4. McShane DJ,
    5. Fries JF,
    6. Cooper NS,
    7. et al.
    The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 1988;31:315–24.
    OpenUrlCrossRefPubMed
  20. 20.↵
    1. Cho YS,
    2. Go MJ,
    3. Kim YJ,
    4. Heo JY,
    5. Oh JH,
    6. Ban HJ,
    7. et al.
    A large-scale genome-wide association study of Asian populations uncovers genetic factors influencing eight quantitative traits. Nat Genet 2009;41:527–34.
    OpenUrlCrossRefPubMed
  21. 21.↵
    1. Prevoo ML,
    2. van ’t Hof MA,
    3. Kuper HH,
    4. van Leeuwen MA,
    5. van de Putte LB,
    6. van Riel PL
    . Modified disease activity scores that include twenty-eight-joint counts. Development and validation in a prospective longitudinal study of patients with rheumatoid arthritis. Arthritis Rheum 1995;38:44–8.
    OpenUrlCrossRefPubMed
  22. 22.↵
    1. Boright AP,
    2. Connelly PW,
    3. Brunt JH,
    4. Morgan K,
    5. Hegele RA
    . Association and linkage of LDLR gene variation with variation in plasma low density lipoprotein cholesterol. J Hum Genet 1998;43:153–9.
    OpenUrlCrossRefPubMed
  23. 23.↵
    1. Benn M,
    2. Nordestgaard BG,
    3. Jensen JS,
    4. Grande P,
    5. Sillesen H,
    6. Tybjaerg-Hansen A
    . Polymorphism in APOB associated with increased low-density lipoprotein levels in both genders in the general population. J Clin Endocrinol Metab 2005;90:5797–803.
    OpenUrlCrossRefPubMed
  24. 24.↵
    1. Kotowski IK,
    2. Pertsemlidis A,
    3. Luke A,
    4. Cooper RS,
    5. Vega GL,
    6. Cohen JC,
    7. et al.
    A spectrum of PCSK9 alleles contributes to plasma levels of low-density lipoprotein cholesterol. Am J Hum Genet 2006;78:410–22.
    OpenUrlCrossRefPubMed
  25. 25.↵
    1. Kathiresan S,
    2. Melander O,
    3. Anevski D,
    4. Guiducci C,
    5. Burtt NP,
    6. Roos C,
    7. et al.
    Polymorphisms associated with cholesterol and risk of cardiovascular events. N Engl J Med 2008;358:1240–9.
    OpenUrlCrossRefPubMed
  26. 26.↵
    1. Sandhu MS,
    2. Waterworth DM,
    3. Debenham SL,
    4. Wheeler E,
    5. Papadakis K,
    6. Zhao JH,
    7. et al.
    LDL-cholesterol concentrations: a genome-wide association study. Lancet 2008;371:483–91.
    OpenUrlCrossRefPubMed
  27. 27.↵
    1. Park MH,
    2. Kim N,
    3. Lee JY,
    4. Park HY
    . Genetic loci associated with lipid concentrations and cardiovascular risk factors in the Korean population. J Med Genet 2011;48:10–5.
    OpenUrlAbstract/FREE Full Text
  28. 28.↵
    1. van der Heijde D
    . How to read radiographs according to the Sharp/van der Heijde method. J Rheumatol 2000;27:261–3.
    OpenUrlPubMed
  29. 29.↵
    1. Saxena R,
    2. Voight BF,
    3. Lyssenko V,
    4. Burtt NP,
    5. de Bakker PI,
    6. Chen H,
    7. et al.
    Genome-wide association analysis identifies loci for type 2 diabetes and triglyceride levels. Science 2007;316:1331–6.
    OpenUrlAbstract/FREE Full Text
  30. 30.↵
    1. Michou L,
    2. Teixeira VH,
    3. Pierlot C,
    4. Lasbleiz S,
    5. Bardin T,
    6. Dieude P,
    7. et al.
    Associations between genetic factors, tobacco smoking and autoantibodies in familial and sporadic rheumatoid arthritis. Ann Rheum Dis 2008;67:466–70.
    OpenUrlAbstract/FREE Full Text
  31. 31.↵
    1. Finckh A,
    2. Dehler S,
    3. Costenbader KH,
    4. Gabay C; and
    5. Swiss Clinical Quality Management project for RA
    . Cigarette smoking and radiographic progression in rheumatoid arthritis. Ann Rheum Dis 2007;66:1066–71.
    OpenUrlAbstract/FREE Full Text
  32. 32.↵
    1. Lindqvist E,
    2. Eberhardt K,
    3. Bendtzen K,
    4. Heinegard D,
    5. Saxne T
    . Prognostic laboratory markers of joint damage in rheumatoid arthritis. Ann Rheum Dis 2005;64:196–201.
    OpenUrlAbstract/FREE Full Text
  33. 33.↵
    1. Matsuzaki T,
    2. Sasaki K,
    3. Hata J,
    4. Hirakawa Y,
    5. Fujimi K,
    6. Ninomiya T,
    7. et al.
    Association of Alzheimer disease pathology with abnormal lipid metabolism: The Hisayama Study. Neurology 2011;77:1068–75.
    OpenUrlCrossRef
  34. 34.↵
    1. Shahar E,
    2. Heiss G,
    3. Rosamond WD,
    4. Szklo M
    . Baldness and myocardial infarction in men: The Atherosclerosis Risk in Communities Study. Am J Epidemiol 2008;167:676–83.
    OpenUrlAbstract/FREE Full Text
  35. 35.↵
    1. Young AR,
    2. Narita M
    . Oncogenic HMGA2: Short or small? Genes Dev 2007;21:1005–9.
    OpenUrlFREE Full Text
  36. 36.↵
    1. Harold D,
    2. Abraham R,
    3. Hollingworth P,
    4. Sims R,
    5. Gerrish A,
    6. Hamshere ML,
    7. et al.
    Genome-wide association study identifies variants at CLU and PICALM associated with Alzheimer’s disease. Nat Genet 2009;41:1088–93.
    OpenUrlCrossRefPubMed
  37. 37.↵
    1. Qi Q,
    2. Liang L,
    3. Doria A,
    4. Hu FB,
    5. Qi L
    . Genetic predisposition to dyslipidemia and type 2 diabetes risk in two prospective cohorts. Diabetes 2012;61:745–52.
    OpenUrlAbstract/FREE Full Text
  38. 38.↵
    1. Purcell SM,
    2. Wray NR,
    3. Stone JL,
    4. Visscher PM,
    5. O’Donovan MC,
    6. Sullivan PF,
    7. et al.
    Common polygenic variation contributes to risk of schizophrenia and bipolar disorder. Nature 2009;460:748–52.
    OpenUrlCrossRefPubMed
  39. 39.↵
    1. Symmons DP,
    2. Bankhead CR,
    3. Harrison BJ,
    4. Brennan P,
    5. Barrett EM,
    6. Scott DG,
    7. et al.
    Blood transfusion, smoking, and obesity as risk factors for the development of rheumatoid arthritis: Results from a primary care-based incident case-control study in Norfolk, England. Arthritis Rheum 1997;40:1955–61.
    OpenUrlCrossRefPubMed
  40. 40.↵
    1. McCarey DW,
    2. McInnes IB,
    3. Madhok R,
    4. Hampson R,
    5. Scherbakov O,
    6. Ford I,
    7. et al.
    Trial of Atorvastatin in Rheumatoid Arthritis (TARA): Double-blind, randomised placebo-controlled trial. Lancet 2004;363:2015–21.
    OpenUrlCrossRefPubMed
  41. 41.↵
    1. Jick SS,
    2. Choi H,
    3. Li L,
    4. McInnes IB,
    5. Sattar N
    . Hyperlipidaemia, statin use and the risk of developing rheumatoid arthritis. Ann Rheum Dis 2009;68:546–51.
    OpenUrlAbstract/FREE Full Text
  42. 42.↵
    1. Gomes AL,
    2. Carvalho T,
    3. Serpa J,
    4. Torre C,
    5. Dias S
    . Hypercholesterolemia promotes bone marrow cell mobilization by perturbing the SDF-1:CXCR4 axis. Blood 2010;115:3886–94.
    OpenUrlAbstract/FREE Full Text
  43. 43.↵
    1. Klaasen R,
    2. Wijbrandts CA,
    3. Gerlag DM,
    4. Tak PP
    . Body mass index and clinical response to infliximab in rheumatoid arthritis. Arthritis Rheum 2011;63:359–64.
    OpenUrlCrossRefPubMed
  44. 44.↵
    1. Tilg H,
    2. Moschen AR
    . Adipocytokines: Mediators linking adipose tissue, inflammation and immunity. Nat Rev Immunol 2006;6:772–83.
    OpenUrlCrossRefPubMed
  45. 45.↵
    1. Tang CH,
    2. Chiu YC,
    3. Tan TW,
    4. Yang RS,
    5. Fu WM
    . Adiponectin enhances IL-6 production in human synovial fibroblast via an AdipoR1 receptor, AMPK, p38, and NF-kappa B pathway. J Immunol 2007;179:5483–92.
    OpenUrlAbstract/FREE Full Text
  46. 46.↵
    1. Ebina K,
    2. Fukuhara A,
    3. Ando W,
    4. Hirao M,
    5. Koga T,
    6. Oshima K,
    7. et al.
    Serum adiponectin concentrations correlate with severity of rheumatoid arthritis evaluated by extent of joint destruction. Clin Rheumatol 2009;28:445–51.
    OpenUrlCrossRefPubMed
  47. 47.↵
    1. Giles JT,
    2. Allison M,
    3. Bingham CO 3rd.,
    4. Scott WM Jr.,
    5. Bathon JM
    . Adiponectin is a mediator of the inverse association of adiposity with radiographic damage in rheumatoid arthritis. Arthritis Rheum 2009;61:1248–56.
    OpenUrlCrossRefPubMed
  48. 48.↵
    1. Newton JL,
    2. Harney SM,
    3. Wordsworth BP,
    4. Brown MA
    . A review of the MHC genetics of rheumatoid arthritis. Genes Immun 2004;5:151–7.
    OpenUrlCrossRefPubMed
PreviousNext
Back to top

In this issue

The Journal of Rheumatology
Vol. 40, Issue 6
1 Jun 2013
  • Table of Contents
  • Table of Contents (PDF)
  • Index by Author
  • Editorial Board (PDF)
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Rheumatology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Association of Polymorphisms Modulating Low-density Lipoprotein Cholesterol with Susceptibility, Severity, and Progression of Rheumatoid Arthritis
(Your Name) has forwarded a page to you from The Journal of Rheumatology
(Your Name) thought you would like to see this page from the The Journal of Rheumatology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Association of Polymorphisms Modulating Low-density Lipoprotein Cholesterol with Susceptibility, Severity, and Progression of Rheumatoid Arthritis
Yune-Jung Park, Seung-Ah Yoo, Susanna Choi, Hee-Soo Yoo, Ho-Sung Yoon, Chul-Soo Cho, Ki-Dong Yoo, Wan-Uk Kim
The Journal of Rheumatology Jun 2013, 40 (6) 798-808; DOI: 10.3899/jrheum.120954

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

 Request Permissions

Share
Association of Polymorphisms Modulating Low-density Lipoprotein Cholesterol with Susceptibility, Severity, and Progression of Rheumatoid Arthritis
Yune-Jung Park, Seung-Ah Yoo, Susanna Choi, Hee-Soo Yoo, Ho-Sung Yoon, Chul-Soo Cho, Ki-Dong Yoo, Wan-Uk Kim
The Journal of Rheumatology Jun 2013, 40 (6) 798-808; DOI: 10.3899/jrheum.120954
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Bookmark this article

Jump to section

  • Article
    • Abstract
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • Appendix 1
    • Appendix 2
    • Appendix 3
    • Appendix 4
    • Appendix 5
    • Appendix 6
    • Footnotes
    • REFERENCES
  • Figures & Data
  • References
  • Info & Metrics
  • PDF
  • eLetters

Keywords

RHEUMATOID ARTHRITIS
DYSLIPIDEMIA
POLYMORPHISMS
RADIOGRAPHIC PROGRESSION

Related Articles

Cited By...

More in this TOC Section

  • Incidence Rates of Psoriasis in Children With Inflammatory Bowel Disease and Juvenile Arthritis Treated With Tumor Necrosis Factor Inhibitors and Disease-Modifying Antirheumatic Drugs
  • COVID-19 vaccine uptake among patients with systemic lupus erythematosus in the American Midwest: The Lupus Midwest Network (LUMEN)
  • Association of M2 Macrophages, Th2, and B Cells With Pathomechanism in Microscopic Polyangiitis Complicated by Interstitial Lung Disease
Show more Articles

Similar Articles

Keywords

  • rheumatoid arthritis
  • DYSLIPIDEMIA
  • POLYMORPHISMS
  • RADIOGRAPHIC PROGRESSION

Content

  • First Release
  • Current
  • Archives
  • Collections
  • Audiovisual Rheum
  • COVID-19 and Rheumatology

Resources

  • Guide for Authors
  • Submit Manuscript
  • Author Payment
  • Reviewers
  • Advertisers
  • Classified Ads
  • Reprints and Translations
  • Permissions
  • Meetings
  • FAQ
  • Policies

Subscribers

  • Subscription Information
  • Purchase Subscription
  • Your Account
  • Terms and Conditions

More

  • About Us
  • Contact Us
  • My Alerts
  • My Folders
  • Privacy/GDPR Policy
  • RSS Feeds
The Journal of Rheumatology
The content of this site is intended for health care professionals.
Copyright © 2022 by The Journal of Rheumatology Publishing Co. Ltd.
Print ISSN: 0315-162X; Online ISSN: 1499-2752
Powered by HighWire