To the Editor:
Intravenous immunoglobulin (IVIG) therapy can benefit diverse autoimmune and inflammatory diseases through several mutually nonexclusive mechanisms1,2. In vitro and in vivo studies in experimental models have also demonstrated that IVIG can expand CD4+CD25+ regulatory T cells (Treg), the cells that play a critical role in maintaining immune tolerance3,4. Treg maintain immune tolerance by suppressing the activation and function of both innate and adaptive immune cells, while deficiency of Treg is associated with autoimmune and inflammatory conditions5,6. Since IVIG therapy in autoimmune patients is associated with restoration of immune tolerance, we hypothesized that this effect of IVIG is in part through expansion of Treg in these patients, the bona fide immune regulators.
We analyzed Treg in paired blood samples of patients with autoimmune rheumatic disease before and 72 to 96 hours after high-dose IVIG therapy (human normal immunoglobulin; Tegeline, 2 g/kg per month)1. The patients broadly belonged to 2 groups: those with idiopathic inflammatory myopathy (8 patients, age range 22–57 yrs; 3 men) and those with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (3 patients, age range 61–68 yrs; 2 males). Patients’ data are provided in Table 1. Local ethical committee approval was obtained for collecting blood samples, and patients provided informed consent.
Peripheral blood mononuclear cells were isolated from the blood samples by Ficoll-density gradient, and CD4+CD25high T cells were analyzed by flow cytometry using fluorescence-conjugated monoclonal antibodies (LSR II; BD Biosciences, Le Pont de Claix, France).
We found that 6 patients, including the 3 with ANCA-associated vasculitis, had substantial increases in the percentage of Treg following IVIG therapy (2.2% ± 0.3% before IVIG therapy and 7.9% ± 1.8% post-IVIG therapy); 3 patients with myopathies (Patients 7, 8, and 11) had marginal enhancement in Treg (1.1% ± 0.6% before IVIG therapy and 1.7% ± 0.7% post-IVIG therapy); and in 2 patients with dermatomyositis (Patients 2 and 6) the percentage of Treg did not change (1.95% ± 0.7% before IVIG therapy and 1.97% ± 0.6% post-IVIG therapy; Figure 1). These results indicate that the antiinflammatory effect of IVIG therapy is associated with enhancement of Treg in autoimmune patients and these Treg might help to restore immune tolerance.
Although several immunosuppressive drugs including steroids can enhance Treg7, IVIG has an added advantage in that this therapy is not an immunosuppressor, but rather an immunomodulator. Hence adverse effects associated with immunosuppressive therapies can be avoided by IVIG therapy. The enhancement of Treg following IVIG therapy might implicate several mutually nonexclusive mechanisms8,9,10. It is known that inflammatory cytokines suppress Treg8 and by neutralizing these inflammatory mediators, IVIG might favor Treg expansion. In addition, IVIG is known to modulate the maturation and function of innate immune cells, and these modulated innate cells may expand Treg. Alternatively, IVIG can reciprocally regulate pathogenic Th17 and Treg10.
Footnotes
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Supported by Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie and Université Paris Descartes, European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 260338 ALLFUN.